Stach C M, Turnay X, Voll R E, Kern P M, Kolowos W, Beyer T D, Kalden J R, Herrmann M
Institute of Clinical Immunology and Rheumatology, Department of Internal Medicine III, Friedrich-Alexander University of Erlangen-Nuernberg, 91054 Erlangen, Germany.
Cell Death Differ. 2000 Oct;7(10):911-5. doi: 10.1038/sj.cdd.4400715.
Exposure of phosphatidylserine on the outer leaflet of the cytoplasmic membrane is an early event during apoptotic cell death and serves as a recognition signal for phagocytes. Usually the clearance of apoptotic cells does not initiate inflammation or immune response. We investigated the immune response in Balb/c mice towards apoptotic human T-cells. Animals injected with apoptotic cells showed significantly reduced humoral immune responses, especially Th1-dependent IgG2a titres, compared to controls immunised with viable cells. However, treatment of apoptotic cells with annexin V (AxV) significantly increased the humoral immune response. AxV binds with high affinity to anionic phospholipids and as a result interferes with the phosphatidylserine recognition by phagocytes. Our results indicate that AxV treatment may be used to increase the efficiency of apoptotic cell-based vaccines, e.g. some tumour vaccines.
磷脂酰丝氨酸暴露于细胞质膜的外小叶是凋亡细胞死亡过程中的早期事件，并作为吞噬细胞的识别信号。通常，凋亡细胞的清除不会引发炎症或免疫反应。我们研究了Balb/c小鼠对凋亡人T细胞的免疫反应。与用活细胞免疫的对照组相比，注射凋亡细胞的动物体液免疫反应显著降低，尤其是Th1依赖的IgG2a滴度。然而，用膜联蛋白V（AxV）处理凋亡细胞可显著增强体液免疫反应。AxV与阴离子磷脂具有高亲和力结合，结果干扰了吞噬细胞对磷脂酰丝氨酸的识别。我们的结果表明，AxV处理可用于提高基于凋亡细胞的疫苗（如某些肿瘤疫苗）的效率。
