Yu Z, Mohn W W
Department of Microbiology and Immunology and Pulp and Paper Centre, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Appl Environ Microbiol. 2001 Apr;67(4):1565-74. doi: 10.1128/AEM.67.4.1565-1574.2001.
We investigated the bacterial community structure in an aerated plug-flow lagoon treating pulp and paper mill effluent. For this investigation, we developed a composite method based on analyses of PCR amplicons containing the ribosomal intergenic spacer (RIS) and its flanking partial 16S rRNA gene. Community percent similarity was determined on the basis of RIS length polymorphism. A community succession was evident in the lagoon, indicated by a progressive community transition through seven sample locations. The most abrupt changes in community structure were associated with a temperature change from 39 to 35 degrees C and with increases in dissolved oxygen. The temporal differences in community structure, based on summer and winter samplings, were greater than the spatial differences during either season. Clone libraries of rDNA-RIS amplicons were constructed from each of three summer samples. Among 90 clones analyzed (30 clones from each sample), 56 phylotypes were distinguished by restriction fragment length polymorphism. Indices of phylotype richness, evenness, and diversity all increased in clone libraries from the beginning to the end of the lagoon. A representative clone of each phylotype was phylogenetically analyzed on the basis of its partial 16S rRNA gene sequence (ca. 450 bp). Phylogenetic analysis confirmed the increase in diversity and further indicated increasing richness of bacterial divisions. Pioneers in the community spatial succession appeared to include thermotolerant, microaerophilic methanol-oxidizing bacteria related to the genus Methylobacillus, as well as thermotolerant, microaerophilic nitrogen-fixing bacteria related to the genus Azospirillum.
我们研究了曝气推流式泻湖处理造纸厂废水过程中的细菌群落结构。为此，我们基于对包含核糖体基因间隔区（RIS）及其侧翼部分16S rRNA基因的PCR扩增子的分析，开发了一种复合方法。群落百分比相似度是根据RIS长度多态性确定的。泻湖中明显存在群落演替，这通过在七个采样点的群落逐渐转变得以体现。群落结构最显著的变化与温度从39摄氏度降至35摄氏度以及溶解氧的增加有关。基于夏季和冬季采样的群落结构时间差异大于任何一个季节内的空间差异。从三个夏季样本中的每一个构建了rDNA - RIS扩增子的克隆文库。在分析的90个克隆（每个样本30个克隆）中，通过限制性片段长度多态性区分出56个系统发育型。从泻湖起点到终点的克隆文库中，系统发育型丰富度、均匀度和多样性指数均有所增加。基于其部分16S rRNA基因序列（约450 bp）对每个系统发育型的代表性克隆进行了系统发育分析。系统发育分析证实了多样性的增加，并进一步表明细菌类群的丰富度在增加。群落空间演替中的先锋菌似乎包括与甲基芽孢杆菌属相关的耐热、微需氧甲醇氧化菌，以及与固氮螺菌属相关的耐热、微需氧固氮菌。
