Biswas S K, Yokoyama K, Wang L, Nishimura K, Miyaji M
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
J Clin Microbiol. 2001 Apr;39(4):1600-3. doi: 10.1128/JCM.39.4.1600-1603.2001.
Including type strains, mitochondrial cytochrome b genes of 32 strains of Candida albicans and 6 strains of Candida stellatoidea, presently treated as a synonym for C. albicans, were partially sequenced. Analysis of 396-bp nucleotide sequences of the strains under investigation divided C. albicans isolates into three types: type I, type II, and type III; however, strains of C. stellatoidea represented distinct type IV isolates. Deduced amino acid sequences of type I, type II, and type III were identical and differed from that of type IV by one amino acid. Genotypes (rDNA type) of the test strains were also checked. Cytochrome b typing did not correlate with genotyping, and different genotypes occurred for one cytochrome b type. This study shows that cytochrome b gene sequences are useful for analyzing the genetic relatedness of C. albicans isolates and effective for differentiating C. stellatoidea from C. albicans.
包括模式菌株在内，对32株白色念珠菌和6株星状念珠菌（目前被视为白色念珠菌的同义词）的线粒体细胞色素b基因进行了部分测序。对所研究菌株396个碱基对的核苷酸序列分析将白色念珠菌分离株分为三种类型：I型、II型和III型；然而，星状念珠菌菌株代表了不同的IV型分离株。I型、II型和III型的推导氨基酸序列相同，与IV型相差一个氨基酸。还检查了测试菌株的基因型（核糖体DNA类型）。细胞色素b分型与基因分型不相关，一种细胞色素b类型出现了不同的基因型。这项研究表明，细胞色素b基因序列可用于分析白色念珠菌分离株的遗传相关性，并有效地将星状念珠菌与白色念珠菌区分开来。
