Yokoyama K, Biswas S K, Miyaji M, Nishimura K
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
J Clin Microbiol. 2000 Dec;38(12):4503-10. doi: 10.1128/JCM.38.12.4503-4510.2000.
We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, and C. lusitaniae. The recently described species of Candida, C. dubliniensis, associated with mucosal candidiasis in human immunodeficiency virus-infected individuals, was also included. Two to five strains of each species were examined. Some species represented intraspecies variation, which was not more than 1.8% (DNA). However, interspecies variations were more than 10 and 7%, respectively, for DNA and amino acid sequences. Multiple alignments of nucleotide and deduced amino acid sequences revealed species-specific nucleotides and amino acids. Nucleotide- and amino acid-based phylogenetic trees were constructed and are discussed. Using the database, it is possible to identify presumptive Candida species within a working day.
我们对临床上最重要的念珠菌属常见物种的线粒体细胞色素b基因的一个396bp区域进行了测序，这些物种包括：白色念珠菌、光滑念珠菌、近平滑念珠菌、热带念珠菌、克柔念珠菌和葡萄牙念珠菌。最近描述的与人类免疫缺陷病毒感染个体的黏膜念珠菌病相关的念珠菌属物种——都柏林念珠菌也包括在内。对每个物种的2至5个菌株进行了检测。一些物种表现出种内变异，其不超过1.8%（DNA）。然而，DNA和氨基酸序列的种间变异分别超过10%和7%。核苷酸和推导的氨基酸序列的多重比对揭示了物种特异性的核苷酸和氨基酸。构建并讨论了基于核苷酸和氨基酸的系统发育树。利用该数据库，有可能在一个工作日内鉴定出推测的念珠菌属物种。
