热带念珠菌、白色念珠菌及其同义词星状念珠菌和克柔念珠菌的DNA相关性、核型分析和基因探测

DNA relatedness, karyotyping and gene probing of Candida tropicalis, Candida albicans and its synonyms Candida stellatoidea and Candida claussenii.

作者信息

Mahrous M, Sawant A D, Pruitt W R, Lott T, Meyer S A, Ahearn D G

机构信息

Laboratory for Microbial and Biochemical Sciences, Georgia State University, Atlanta 30303.

出版信息

Eur J Epidemiol. 1992 May;8(3):444-51. doi: 10.1007/BF00158581.

DOI:10.1007/BF00158581

PMID:1397209

Abstract

Isolates of Candida albicans with varied phenotypes, including sucrose-negative variants (C. stellatoidea, serotypes A and B) and avirulent germ tube-negative forms (C. claussenii) showed significant (greater than 90%) DNA relatedness to classical C. albicans, but insignificant relatedness to C. tropicalis and sucrose-negative C. tropicalis. A transverse alternating-field gel electrophoresis procedure (TAFE) showed discrete karyotype patterns among the phenotypic variants of C. albicans including the sucrose-negative C. stellatoidea. The number of chromosome-sized DNA bands for C. tropicalis (7 bands) were within the range of bands observed for C. albicans (5 to 10 bands). The general DNA-migration pattern for C. albicans appeared distinct from that of C. tropicalis. An aspartyl proteinase (PrA) gene probe from C. albicans hybridized with chromosomal DNA from C. albicans, C. claussenii and C. stellatoidea but not with that from C. tropicalis.

摘要

白色念珠菌的不同表型分离株，包括蔗糖阴性变体（星状念珠菌，血清型A和B）和无毒力的芽管阴性形态（克劳森念珠菌），与经典白色念珠菌显示出显著的（大于90%）DNA相关性，但与热带念珠菌和蔗糖阴性热带念珠菌的相关性不显著。横向交变电场凝胶电泳程序（TAFE）显示，白色念珠菌的表型变体（包括蔗糖阴性星状念珠菌）之间具有离散的核型模式。热带念珠菌的染色体大小DNA条带数量（7条带）在白色念珠菌观察到的条带范围内（5至10条带）。白色念珠菌的一般DNA迁移模式与热带念珠菌明显不同。来自白色念珠菌的天冬氨酸蛋白酶（PrA）基因探针与白色念珠菌、克劳森念珠菌和星状念珠菌的染色体DNA杂交，但不与热带念珠菌的染色体DNA杂交。