Müller A, Bialek R, Kämper A, Fätkenheuer G, Salzberger B, Franzen C
Department of Pediatrics, University of Cologne, Cologne, Germany.
J Clin Microbiol. 2001 Apr;39(4):1630-2. doi: 10.1128/JCM.39.4.1630-1632.2001.
We examined stool specimens of 148 returning travelers from an outpatient department for tropical diseases for the appearance of microsporidia using light microscopy and PCR. Intestinal microsporidiosis was diagnosed for five patients by light microscopy and for nine patients by PCR. Some cases were diagnosed only by PCR, indicating that the true prevalence has to be determined by highly sensitive techniques, such as PCR.
我们利用光学显微镜和聚合酶链反应(PCR),对一家热带病门诊部门的148名归国旅行者的粪便样本进行了微孢子虫外观检查。通过光学显微镜诊断出5例肠道微孢子虫病患者,通过PCR诊断出9例。有些病例仅通过PCR得以诊断,这表明必须采用诸如PCR这样的高灵敏度技术来确定实际患病率。