骨骼肌萎缩患者人成纤维细胞中非特异性酯酶和酸性磷酸酶的定位
Localization of non-specific esterase and acid phosphatase in human fibroblast from skeletal muscle atrophy.
作者信息
Ohyumi M, Ohyumi M
出版信息
Acta Histochem. 1979;64(1):98-105. doi: 10.1016/S0065-1281(79)80058-6.
The intracellular localization of non-specific esterase and acid phosphatase was investigated in human fibroblast cells from skeletal muscle atrophy. Non-specific esterase and acid phosphatase positive sites were visualized ultrastructurally in the fibroblast. Electron microscopy for the cytochemistry of these enzyme was performed in human atrophic skeletal muscle by using thiol acetate esterase method and GOMORI'S method. Lipofuscin pigment granules in fibroblast cells contain dense pigment, granular matrix and lipid droplet. Reaction products of non-specific esterase are seen in the pigment and granular matrix, and they may therefore be called residual bodies. Reaction products of acid phosphatase and non-specific esterase were found to be located in lysosomes.
对来自骨骼肌萎缩患者的人成纤维细胞中非特异性酯酶和酸性磷酸酶的细胞内定位进行了研究。在成纤维细胞中通过超微结构观察到非特异性酯酶和酸性磷酸酶阳性位点。利用硫醇乙酸酯酶法和GOMORI法对人萎缩性骨骼肌中的这些酶进行细胞化学电子显微镜检查。成纤维细胞中的脂褐素色素颗粒含有致密色素、颗粒状基质和脂滴。在色素和颗粒状基质中可见非特异性酯酶的反应产物，因此它们可能被称为残余小体。发现酸性磷酸酶和非特异性酯酶的反应产物位于溶酶体中。
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