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实验性牛囊尾蚴病不同发育阶段碱性磷酸酶、酸性磷酸酶及非特异性酯酶的活性

The activity of alkaline and acid phosphatase and non-specific esterase in experimental bovine cysticercosis at different stages of development.

作者信息

Zdárská Z, Blazek K, Schramlová J

出版信息

Folia Parasitol (Praha). 1981;28(1):55-9.

PMID:7227869
Abstract

The activity of alkaline and acid phosphatase and non-specific esterase was detected both in the parasite and in the tissue reaction on days 21, 23, 42, 168 and 261 after experimental infection. A very high activity of all enzymes was found in 21- and 23-day-old C. bovis in the tegument of whole bladder. In 42, 168 and 261 days old cysticerci the activity of alkaline acid phosphatase was limited only to a part of bladder surrounding the opening of the spinal canal, whereas the activity of non-specific esterase was present in the whole bladder. The activity of non-specific esterase was localized in subtegumental cells of the bladder wall and in small bodies in the bladder and scolex. These bodies increased in number with the age of the cysticercus. In the tissue reaction, a high activity of alkaline phosphatase was detected only in the period of about 20 days after infection in the layer of activated fibroblasts. The activity of acid phosphatase was demonstrated in the tissue reaction in all time periods and was localized in the histiocytes, macrophages, necrotic exudate, necrotic foci and pigment cells at the periphery of tissue reaction. These cells exhibited also the activity of non-specific esterase.

摘要

在实验感染后的第21天、23天、42天、168天和261天,检测了寄生虫以及组织反应中碱性磷酸酶、酸性磷酸酶和非特异性酯酶的活性。在21日龄和23日龄的牛囊尾蚴整个囊壁的皮层中发现所有酶的活性都非常高。在42日龄、168日龄和261日龄的囊尾蚴中,碱性磷酸酶的活性仅局限于围绕椎管开口的部分囊壁,而非特异性酯酶的活性则存在于整个囊壁中。非特异性酯酶的活性定位于囊壁的皮层下细胞以及囊壁和头节中的小体。这些小体的数量随着囊尾蚴的年龄增长而增加。在组织反应中,仅在感染后约20天的时期内,在活化成纤维细胞层中检测到碱性磷酸酶的高活性。酸性磷酸酶的活性在所有时间段的组织反应中均有表现,且定位于组织反应周边的组织细胞、巨噬细胞、坏死渗出物、坏死灶和色素细胞中。这些细胞也表现出非特异性酯酶的活性。

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