Cardoso D D, Rácz M L, Azevedo M S, Martins R M, Soares C M
Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua Delenda Rezende Melo, Esquina com 1a Avenida, 74605-050 Goiânia, GO, Brazil.
Braz J Med Biol Res. 2001 Apr;34(4):471-3. doi: 10.1590/s0100-879x2001000400005.
The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. The knowledge of genotypes provides important information concerning rotavirus circulation in Central Brazil.
使用针对人轮状病毒血清型/基因型1、2、3和4的VP7基因区域的寡核苷酸探针，通过RNA-DNA杂交（斑点杂交）对74份A组轮状病毒样本进行G基因分型。31份样本可通过斑点杂交进行基因分型，结果如下：G1 = 16，G4 = 6，G3 = 5，G2 = 4。数据显示基因型G1-G4的流行情况以及G1的优势地位。基因型的相关知识为巴西中部轮状病毒的传播提供了重要信息。
