Tasca T, De Carli G A, Glock L, Jeckel-Neto E A
Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, 90619-900, Brasil.
Mem Inst Oswaldo Cruz. 2001 Feb;96(2):265-71. doi: 10.1590/s0074-02762001000200022.
Tetratrichomonas didelphidis (Hegner & Ratcliffe, 1927) Andersen & Reilly, 1965 is a flagellate protozoan found in the intestine, cecum, and colon of Didelphis marsupialis. The parasitic protozoa used in this study was found and isolated in the intestine of opossums in Pavlova starch-containing medium in Florianópolis, State of Santa Catarina, Brazil, from D. marsupialis and Lutreolina crassicaudata. The strains were cultivated in Diamond medium without maltose and with starch solution, pH 7.5 at 28 degrees C. The specimens were stained by the Giemsa method and Heidenhain's iron hematoxylin. The light microscopy study of the trophozoites revealed the same morphologic characteristics as specimens previously described.
双门齿四毛滴虫（赫格纳和拉特克利夫，1927年）安徒生和赖利，1965年，是一种鞭毛虫原生动物，发现于袋鼩的肠道、盲肠和结肠中。本研究中使用的寄生原生动物是在巴西南里奥格兰德州弗洛里亚诺波利斯的含有帕夫洛娃淀粉的培养基中，从袋鼩和粗尾水獭的肠道中发现并分离出来的。这些菌株在不含麦芽糖但含有淀粉溶液、pH值为7.5、温度为28摄氏度的戴蒙德培养基中培养。标本用吉姆萨法和海登海因氏铁苏木精染色。对滋养体的光学显微镜研究揭示了与先前描述的标本相同的形态特征。
