[人子宫平滑肌瘤中孕激素受体A和B亚型的蛋白表达]

[Protein expression of human progesterone receptor isoforms A and B in uterine leiomyoma].

作者信息

Tu Z, Wei L, Wang J

机构信息

Department of Gynecology, Peking University People's Hospital, Beijing 100044, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2000 Dec;35(12):740-5.

PMID:11286035

Abstract

OBJECTIVE

To investigate the role of two isoforms of human progesterone receptor A (hPR-A) and B (hPR-B) in the development of uterine leiomyoma, their distribution and expression in leiomyoma were detected.

METHODS

The tissues of leiomyoma and normal myometrium from 30 uteri, which were excised for leiomyoma, were used for the localization and quantification of protein of the two isoforms. Immunohistochemistry and Western blot were applied respectively.

RESULTS

Both hPR-A and hPR-B were nuclear receptors. Concentrations of hPR-(A + B) and hPR-A in leiomyoma were higher than those in normal myometrium (295,796 +/- 90,856, 256,275 +/- 98,560; P = 0.042, P = 0.000,563). Both isoforms were presented in leiomyoma and normal myometrium, with a consistent dominance of hPR-B over hPR-A. More expression of hPR-A was found during secretive phase than proliferative phase, not only in leiomyoma but also in normal myometrium (P = 0.037, P = 0.024).

CONCLUSIONS

The development of uterine leiomyoma seems to be related with the progesterone receptor isoforms, especially hPR-A.

摘要

目的

研究人孕激素受体A（hPR-A）和B（hPR-B）的两种亚型在子宫平滑肌瘤发生发展中的作用，检测它们在平滑肌瘤中的分布及表达情况。

方法

选取30例因平滑肌瘤而切除子宫的组织，取其中的平滑肌瘤组织和正常子宫肌层组织，用于两种亚型蛋白的定位和定量分析，分别采用免疫组织化学法和蛋白质印迹法。

结果

hPR-A和hPR-B均为核受体。平滑肌瘤中hPR-(A + B)和hPR-A的浓度高于正常子宫肌层（295,796±90,856，256,275±98,560；P = 0.042，P = 0.000,563）。两种亚型均在平滑肌瘤和正常子宫肌层中表达，且hPR-B始终占主导地位。在分泌期，hPR-A在平滑肌瘤和正常子宫肌层中的表达均高于增殖期（P = 0.037，P = 0.024）。