孕激素受体同工型 A 和 B、p53 和 p21 在子宫肌层和子宫肌瘤中的基因和蛋白表达。
Gene and protein expression of progesterone receptor isoforms A and B, p53 and p21 in myometrium and uterine leiomyoma.
机构信息
Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
Arch Gynecol Obstet. 2012 Jul;286(1):119-24. doi: 10.1007/s00404-012-2245-2. Epub 2012 Feb 17.
PURPOSE
To assess gene and protein expression of progesterone receptor isoforms A and B, cell cycle regulators p53 and p21 in leiomyoma and myometrium.
METHODS
Samples were collected from 14 patients in reproductive age who underwent abdominal hysterectomy. Gene expression of PRA, PRB, p53 and p21 was analyzed by real-time PCR. Protein expression was assessed by Western blots.
RESULTS
There was no change in gene and protein expression of PRA and PRB in both tissues. The ratio between isoforms (PRA:PRB) was not different between tissues and showed a strong correlation (r = 0.767, P = 0.004). The analysis of gene expression and protein showed increased levels of mRNA and protein p53 in leiomyoma compared to myometrium (P = 0.030 and P = 0.002, respectively). The same increase was observed in p21 mRNA levels (P = 0.016) and protein p21 levels (P = 0.026) in samples of uterine leiomyoma.
CONCLUSIONS
PRA:PRB ratio is similar in normal myometrium and leiomyomas. p53 and p21 mRNA and protein levels are increased in leiomyomas.
目的
评估孕激素受体 A 和 B 亚型、细胞周期调节剂 p53 和 p21 在子宫肌瘤和子宫肌层中的基因和蛋白表达。
方法
收集 14 名处于生育期的接受腹式子宫切除术患者的样本。通过实时 PCR 分析 PRA、PRB、p53 和 p21 的基因表达。通过 Western blot 评估蛋白表达。
结果
在两种组织中，PRA 和 PRB 的基因和蛋白表达均无变化。两种组织之间的亚型（PRA:PRB）比例没有差异，且相关性很强（r = 0.767，P = 0.004）。基因表达和蛋白分析显示，与子宫肌层相比，子宫肌瘤中 p53 的 mRNA 和蛋白水平升高（P = 0.030 和 P = 0.002）。在子宫肌瘤样本中，p21 mRNA 水平（P = 0.016）和蛋白 p21 水平（P = 0.026）也观察到相同的升高。
结论
在正常子宫肌层和子宫肌瘤中，PRA:PRB 比例相似。p53 和 p21 的 mRNA 和蛋白水平在子宫肌瘤中升高。
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