Zabicka D, Młynarczyk G, Łuczak M
Katedra i Zakład Mikrobiologii Lekarskiej, Centrum Biostruktury Akademii Medycznej w Warszawie.
Med Dosw Mikrobiol. 2000;52(4):317-26.
The ability of lysogenization was examined of 50 S. intermedius strains and of 77 strains belonging to 14 different species of coagulase-negative staphylococci using 8 enterotoxin A converting bacteriophages isolated from S. aureus. All the examined bacteriophages showed lytic activity against at least 1 of 11 susceptible strains of S. intermedius to them. Lytic activity towards coagulase-negative staphylococci was observed for 6 of 8 examined bacteriophages. Two bacteriophages were active against 1 of 9 examined S. capitis strains, one against 1 of 11 examined S. haemolyticus strains, four against 1 of 6 examined S. lugdunensis strains, three against 1 of 6 examined S. warneri strains and one against 1 of 5 examined S. xylosus strains. Lysogenization with bacteriophage f421-1 able to convert positively enterotoxin A and staphylokinase and negatively beta-haemolysin of one S. intermedius strain was successful. S. intermedius lysogenized with phi 421-1 was able to produce both enterotoxin A and staphylokinase and lost ability to produce beta-haemolysin. Our results showed a broad lytic spectrum and interspecies host range of some S. aureus bacteriophages and the ability of interspecies transfer of bacteriophages between S. aureus and S. intermedius.
使用从金黄色葡萄球菌中分离出的8种肠毒素A转化噬菌体，检测了50株中间葡萄球菌菌株以及属于14种不同凝固酶阴性葡萄球菌的77株菌株的溶原化能力。所有检测的噬菌体对11株对它们敏感的中间葡萄球菌菌株中的至少1株显示出裂解活性。在检测的8种噬菌体中，有6种对凝固酶阴性葡萄球菌具有裂解活性。两种噬菌体对9株检测的头状葡萄球菌菌株中的1株有活性，一种对11株检测的溶血葡萄球菌菌株中的1株有活性，四种对6株检测的路邓葡萄球菌菌株中的1株有活性，三种对6株检测的沃纳葡萄球菌菌株中的1株有活性，一种对5株检测的木糖葡萄球菌菌株中的1株有活性。用能够正向转化肠毒素A和葡萄球菌激酶且负向转化β-溶血素的噬菌体f421-1对一株中间葡萄球菌进行溶原化成功。用φ421-1溶原化的中间葡萄球菌能够产生肠毒素A和葡萄球菌激酶，并且失去了产生β-溶血素的能力。我们的结果显示了一些金黄色葡萄球菌噬菌体具有广泛的裂解谱和种间宿主范围，以及噬菌体在金黄色葡萄球菌和中间葡萄球菌之间进行种间转移的能力。
