Kumagai Rina, Nakatani Kazue, Ikeya Nanami, Kito Yukiko, Kaidoh Toshio, Takeuchi Shotaro
Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, 4-1-1 Kenjyojima Masuoka, Eiheiji, Fukui 910-0095, Japan.
Vet Microbiol. 2007 May 16;122(1-2):190-5. doi: 10.1016/j.vetmic.2007.01.008. Epub 2007 Jan 17.
In 13 of 43 non-beta-hemolysin-producing bovine isolates of Staphylococcus aureus, two truncated beta-hemolysin (hlb) genes were demonstrated by PCR and sequencing, and one truncated hlb gene was located beside the integrase (int) gene of phage origin. The staphylokinase (sak) gene was detected in all 13 isolates in which the truncated hlb genes were detected by PCR. Enterotoxin A (sea) and enterotoxin P (sep) genes were also detected in 5 and 2 of the 13 isolates, respectively. Moreover, the scn and chp genes encoding staphylococcal complement inhibitor (SCIN) and chemotaxis inhibitory protein of S. aureus (CHIPS) were detected in 13 and 4 of the 13 isolates, respectively. The bacteriophage induced by mitomycin C treatment was able to lysogenize one beta-hemolysin-producing isolate of S. aureus, and the sak and scn genes were detected from the lysogenized isolate. These results suggest quadruple or quintuple conversion of hlb, sak, sea (or sep), scn, and chp genes by bacteriophages among non-beta-hemolysin-producing bovine isolates of S. aureus.
在43株不产生β - 溶血素的金黄色葡萄球菌牛源分离株中，有13株通过聚合酶链反应（PCR）和测序证实存在两个截短的β - 溶血素（hlb）基因，且其中一个截短的hlb基因位于噬菌体来源的整合酶（int）基因旁边。在通过PCR检测到截短hlb基因的所有13株分离株中均检测到葡萄球菌激酶（sak）基因。在这13株分离株中，分别有5株和2株检测到肠毒素A（sea）基因和肠毒素P（sep）基因。此外，在这13株分离株中，分别有13株和4株检测到编码葡萄球菌补体抑制剂（SCIN）的scn基因和金黄色葡萄球菌趋化抑制蛋白（CHIPS）的chp基因。丝裂霉素C处理诱导产生的噬菌体能够使一株产生β - 溶血素的金黄色葡萄球菌分离株发生溶原化，并且从溶原化的分离株中检测到了sak和scn基因。这些结果表明，在不产生β - 溶血素的金黄色葡萄球菌牛源分离株中，噬菌体可导致hlb、sak、sea（或sep）、scn和chp基因发生四重或五重转化。
