Le Floc'h N, Lahaye L, Mézière N, Sève B
Unité Mixte de Recherches sur le Veau et le Porc, INRA, Saint-Gilles, France.
Reprod Nutr Dev. 2000 Nov-Dec;40(6):549-58. doi: 10.1051/rnd:2000136.
Contributions of erythrocytes and plasma to threonine and lysine transport across the PDV and the liver were determined in growing pigs successively fed a threonine deficient diet and a control well-balanced diet (experiment 1) or a lysine deficient or a well-balanced diet (experiment 2). The animals were surgically prepared for insertion of chronic catheters in the mesenteric vein (MV), the portal vein (PV), a hepatic vein (HV) and the carotid artery (CA). Plasma and whole blood AA concentrations in PV, HV and CA and PV and HV blood flows were determined during 6 hours of para-aminohippuric acid constant infusion. During this period the pigs were continuously fed (1 meal per hour). The contribution of plasma to lysine and threonine transport was higher in pigs fed the well balanced diets. More than 50% of threonine and lysine appearing in the PV and in the HV are transported by the plasma. Our results suggest that erythrocytes are probably little involved in lysine and threonine transfer across the liver and digestive tract of pig continuously fed.
在生长猪中，先后给它们饲喂苏氨酸缺乏日粮和对照平衡日粮（实验1）或赖氨酸缺乏日粮或平衡日粮（实验2），以确定红细胞和血浆对苏氨酸和赖氨酸跨肠-门静脉组织（PDV）和肝脏转运的贡献。通过手术给动物准备好，以便在肠系膜静脉（MV）、门静脉（PV）、肝静脉（HV）和颈动脉（CA）中插入慢性导管。在对氨基马尿酸持续输注6小时期间，测定PV、HV和CA中的血浆和全血氨基酸浓度以及PV和HV的血流。在此期间，持续给猪喂食（每小时一餐）。在饲喂平衡日粮的猪中，血浆对赖氨酸和苏氨酸转运的贡献更高。出现在PV和HV中的苏氨酸和赖氨酸，超过50%是由血浆转运的。我们的结果表明，在持续喂食的猪中，红细胞可能很少参与赖氨酸和苏氨酸跨肝脏和消化道的转运。
