用于检测沙门氏菌属的抗体固定化石英晶体微天平系统的操作特性
Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp.
作者信息
Park I S, Kim W Y, Kim N
机构信息
Korea Food Research Institute, Kyungki-do, Republic of Korea.
出版信息
Biosens Bioelectron. 2000 Jun;15(3-4):167-72. doi: 10.1016/s0956-5663(00)00053-1.
A quartz crystal microbalance (QCM) system detecting Salmonella spp. was developed by an anti-Salmonella antibody immobilization onto one gold surface of a piezoelectric quartz crystal surface with sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) thiolation. The optimum temperature and pH for the antibody-immobilized sensor were 35 degrees C and 7.2, respectively. The frequency shifts obtained were correlated with the Salmonella concentrations in the range 3.2 x 10(6)-4.8 x 10(8) CFU per ml. The system was quite specific to Salmonella spp. and applicable for repetitive use after a regeneration step employing 1.2 M NaOH. A model sample measurement was done for a market milk spiked with Salmonella typhimurium.
通过用磺基琥珀酰亚胺6-[3-(2-吡啶二硫基)丙酰胺基]己酸酯（磺基-LC-SPDP）硫醇化将抗沙门氏菌抗体固定在压电石英晶体表面的一个金表面上，开发了一种检测沙门氏菌属的石英晶体微天平（QCM）系统。固定有抗体的传感器的最佳温度和pH分别为35℃和7.2。获得的频率变化与每毫升3.2×10⁶ - 4.8×10⁸ CFU范围内的沙门氏菌浓度相关。该系统对沙门氏菌属具有很高的特异性，并且在采用1.2 M NaOH的再生步骤后可重复使用。对添加了鼠伤寒沙门氏菌的市售牛奶进行了模型样品测量。
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