Wing J P, Schwartz L M, Nambu J R
Molecular and Cellular Biology Program, University of Massachusetts at Amherst, 01003, USA.
Mech Dev. 2001 Apr;102(1-2):193-203. doi: 10.1016/s0925-4773(01)00316-1.
Reaper, Hid, and Grim are three Drosophila cell death activators that each contain a conserved NH(2)-terminal Reaper, Hid, Grim (RHG) motif. We have analyzed the importance of the RHG motifs in Reaper and Grim for their different abilities to activate cell death during development. Analysis of chimeric R/Grim and G/Reaper proteins indicated that the Reaper and Grim RHG motifs are functionally distinct and help to determine specific cell death activation properties. A truncated GrimC protein lacking the RHG motif retained an ability to induce cell death, and unlike Grim, R/Grim, or G/Reaper, its actions were not efficiently blocked by the cell death inhibitors, Diap1, Diap2, p35, or a dominant/negative Dronc caspase. Finally, we identified a second region of sequence similarity in Reaper, Hid, and Grim, that may be important for shared RHG motif-independent activities.
收割者（Reaper）、希德（Hid）和格里姆（Grim）是三种果蝇细胞死亡激活因子，它们各自都含有一个保守的氨基末端收割者、希德、格里姆（RHG）基序。我们分析了收割者和格里姆中RHG基序对于它们在发育过程中激活细胞死亡的不同能力的重要性。对嵌合的R/格里姆和G/收割者蛋白的分析表明，收割者和格里姆的RHG基序在功能上是不同的，有助于确定特定的细胞死亡激活特性。一种缺少RHG基序的截短型格里姆C蛋白保留了诱导细胞死亡的能力，并且与格里姆、R/格里姆或G/收割者不同，其作用不能被细胞死亡抑制剂DIAP1、DIAP2、p35或显性/阴性Dronc半胱天冬酶有效阻断。最后，我们在收割者、希德和格里姆中鉴定出了第二个序列相似区域，这可能对于不依赖RHG基序的共同活性很重要。
