Department of Neurobiology, Department of Neurology of Sir Run Run Shaw Hospital and School of Brain Science and Brain Medicine, Zhejiang University School of Medicine, Hangzhou, 310058, China.
MOE Frontier Science Center for Brain Research and Brain-Machine Integration, State Key Laboratory of Brain-machine Intelligence, Zhejiang University, 1369 West Wenyi Road, Hangzhou, 311121, China.
Nat Commun. 2024 Apr 2;15(1):2834. doi: 10.1038/s41467-024-47278-5.
The circadian clock regulates animal physiological activities. How temperature reorganizes circadian-dependent physiological activities remains elusive. Here, using in-vivo two-photon imaging with the temperature control device, we investigated the response of the Drosophila central circadian circuit to temperature variation and identified that DN1as serves as the most sensitive temperature-sensing neurons. The circadian clock gate DN1a's diurnal temperature response. Trans-synaptic tracing, connectome analysis, and functional imaging data reveal that DN1as bidirectionally targets two circadian neuronal subsets: activity-related E cells and sleep-promoting DN3s. Specifically, behavioral data demonstrate that the DN1a-E cell circuit modulates the evening locomotion peak in response to cold temperature, while the DN1a-DN3 circuit controls the warm temperature-induced nocturnal sleep reduction. Our findings systematically and comprehensively illustrate how the central circadian circuit dynamically integrates temperature and light signals to effectively coordinate wakefulness and sleep at different times of the day, shedding light on the conserved neural mechanisms underlying temperature-regulated circadian physiology in animals.
生物钟调节动物的生理活动。温度如何重新组织生物钟依赖性的生理活动仍然难以捉摸。在这里，我们使用具有温度控制装置的体内双光子成像，研究了果蝇中央生物钟回路对温度变化的反应，并确定 DN1as 作为最敏感的温度感应神经元。生物钟门控 DN1a 的昼夜温度反应。转突触示踪、连接组分析和功能成像数据显示，DN1as 双向靶向两个生物钟神经元亚群：与活动相关的 E 细胞和促进睡眠的 DN3s。具体来说，行为数据表明，DN1a-E 细胞回路调节对冷温度的傍晚运动峰值，而 DN1a-DN3 回路控制夜间睡眠减少的温暖温度诱导。我们的研究结果系统而全面地说明了中央生物钟回路如何动态整合温度和光信号，有效地协调一天中不同时间的觉醒和睡眠，为动物中受温度调节的生物钟生理学的保守神经机制提供了线索。
