一种用于测定组织和孵育培养基中标记去甲肾上腺素及其代谢产物的薄层色谱法。

A thin-layer chromatographic procedure for the assay of labelled noradrenaline and its metabolites in tissues and in incubation medium.

作者信息

Vohra M M, Jayasundar S

出版信息

J Pharm Pharmacol. 1976 Nov;28(11):810-4. doi: 10.1111/j.2042-7158.1976.tb04062.x.

DOI:10.1111/j.2042-7158.1976.tb04062.x

Abstract

A method for the extraction, separation and quantitative determination of [3H]noradrenaline [3H-NA] and its five major metabolites has been devised using thin layer chromatography. This procedure was used to study the pattern of 3H-NA and its metabolites in the total radioactivity of the tissues and that released spontaneously from the rat isolated vas deferens. Whereas in tissues 3H-NA represented almost all of the total radioactivity (94-8+/-0.47%), in the samples of spontaneous outflow it represented only 16-8+/-2.1%. The rest was mostly accounted for by the five metabolites, primarily 3H-DOPEG and 3H-MOPEG. These findings show that in the rat vas deferens 3H-NA is preferentially metabolized via the two glycol derivatives, i.e. 3H-DOPEG and 3H-MOPEG.

摘要

已设计出一种使用薄层色谱法提取、分离和定量测定[3H]去甲肾上腺素[3H-NA]及其五种主要代谢物的方法。该程序用于研究3H-NA及其代谢物在组织总放射性以及从大鼠离体输精管中自发释放的放射性中的分布模式。在组织中，3H-NA几乎占总放射性的全部（94.8±0.47%），而在自发流出物样本中，它仅占16.8±2.1%。其余大部分由五种代谢物构成，主要是3H-DOPEG和3H-MOPEG。这些发现表明，在大鼠输精管中，3H-NA优先通过两种二醇衍生物即3H-DOPEG和3H-MOPEG进行代谢。