Iwai M, Tamai S, Yajima H, Kawanishi K
Department of Orthopaedic Surgery, Saiseikai Nara Hospital, Nara-shi, Nara, Japan.
Microsurgery. 2001;21(2):43-51. doi: 10.1002/micr.1007.
A comparative study of nerve regeneration was performed on vascularized nerve graft (VNG) and free nerve graft (FNG) in Fischer strain rats. A segment of the sciatic nerve with vascular pedicle of the femoral artery and vein was harvested from syngeneic donor rat for the VNG group and the sciatic nerve in the same length without vascular pedicle was harvested for the FNG group. They were transplanted to a nerve defect in the sciatic nerve of syngeneic recipient rats. At 2, 4, 6, 8, 12, 16, and 24 weeks after operation, the sciatic nerves were biopsied and processed for evaluation of choline acetyltransferase (CAT) activity, histological studies, and measurement of wet weight of the muscle innervated by the sciatic nerve. Electrophysiological evaluation of the grafted nerve was also performed before sacrifice. The average CAT activity in the distal to the distal suture site was 383 cpm in VNG and 361 cpm in FNG at 2 weeks; 6,189 cpm in VNG and 2,264 cpm in FNG at 4 weeks; and 11,299 cpm in VNG and 9,424 cpm in FNG at 6 weeks postoperatively. The value of the VNG group was statistically higher than that of the FNG group at 4 weeks postoperatively. Electrophysiological and histological findings also suggested that nerve regeneration in the VNG group was superior to that in the FNG group during the same period. However, there was no significant difference between the two groups after 6 weeks postoperatively in any of the evaluations. The CAT measurement was useful in the experiments, because it was highly sensitive and reproducible.
在Fischer品系大鼠中对带血管神经移植（VNG）和游离神经移植（FNG）进行了神经再生的比较研究。从同基因供体大鼠获取一段带有股动脉和静脉血管蒂的坐骨神经用于VNG组，获取相同长度无血管蒂的坐骨神经用于FNG组。将它们移植到同基因受体大鼠坐骨神经的缺损处。术后2、4、6、8、12、16和24周，对坐骨神经进行活检并处理，以评估胆碱乙酰转移酶（CAT）活性、进行组织学研究以及测量由坐骨神经支配的肌肉的湿重。在处死前还对移植神经进行了电生理评估。术后2周时，VNG组远端至远端缝合部位的平均CAT活性为383 cpm，FNG组为361 cpm；术后4周时，VNG组为6189 cpm，FNG组为2264 cpm；术后6周时，VNG组为11299 cpm，FNG组为9424 cpm。术后4周时，VNG组的值在统计学上高于FNG组。电生理和组织学结果也表明，同期VNG组的神经再生优于FNG组。然而，术后6周后，在任何一项评估中两组之间均无显著差异。CAT测量在实验中很有用，因为它高度敏感且可重复。
