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采用基于合成肽的免疫分析法测定牛群中绵羊和牛呼吸道合胞体病毒感染的流行情况。

Prevalence of ovine and bovine respiratory syncytial virus infections in cattle determined with a synthetic peptide-based immunoassay.

作者信息

Grubbs S T, Kania S A, Potgieter L N

机构信息

Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071, USA.

出版信息

J Vet Diagn Invest. 2001 Mar;13(2):128-32. doi: 10.1177/104063870101300206.

DOI:10.1177/104063870101300206
PMID:11289208
Abstract

Subgroup-specific peptide-based enzyme-linked immunosorbent assays from the G-protein of the ovine and bovine respiratory syncytial virus (RSV), respectively, were used to determine the prevalence of the ovine and bovine subgroup strains of RSV infections in cattle. A total of 1,102 bovine serum samples were obtained from 6 diagnostic laboratories located in the northwestern and the southeastern USA and were tested for antibody to either the bovine or ovine subgroups of RSV. Antibody to viruses from each subgroup was present in samples from each region and all states tested. The Southeast had a higher prevalence of the bovine subgroup strains (69.5%). Then did the Northwest (40.9%). The prevalence of the ovine strain was similar for the two regions (16.7% in the southeast, 14.9% in the northwest). The overall prevalence was 56.6% for the bovine strain and 15.9% for the ovine strain. These results suggest members of the ovine subgroup of RSV circulate in the cattle population but with less frequency than those viruses of the bovine subgroup.

摘要

分别使用基于绵羊和牛呼吸道合胞病毒(RSV)G蛋白的亚组特异性肽酶联免疫吸附试验,来确定牛群中绵羊亚组和牛亚组RSV感染毒株的流行情况。从位于美国西北部和东南部的6个诊断实验室共采集了1102份牛血清样本,并检测其针对RSV牛亚组或绵羊亚组的抗体。来自每个地区以及所有检测州的样本中均存在针对每个亚组病毒的抗体。东南部牛亚组毒株的流行率较高(69.5%),西北部(40.9%)次之。两个地区绵羊毒株的流行率相似(东南部为16.7%,西北部为14.9%)。牛毒株的总体流行率为56.6%,绵羊毒株为15.9%。这些结果表明,RSV绵羊亚组的毒株在牛群中传播,但频率低于牛亚组病毒。

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