Lahmann C, Bergemann J, Harrison G, Young A R
Lancet. 2001 Mar 24;357(9260):935-6. doi: 10.1016/S0140-6736(00)04220-3.
Smokers look older than non-smokers of the same age. We have compared the concentrations of mRNA for matrix metalloproteinase 1 (MMP-1) in the buttock skin of smokers and non-smokers with quantitative real-time polymerase chain reactions. MMP-1 degrades collagen, which accounts for at least 70% of the dry weight of dermis. We report significantly more MMP-1 mRNA in the skin of smokers than non-smokers whereas no difference was seen for the tissue inhibitor of metalloproteinases 1 (TIMP-1) or the housekeeping gene GAPDH (glyceraldehyde-3-phosphate dehydrogenase). We suggest that smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking.
吸烟者看起来比同龄的非吸烟者更显老。我们通过定量实时聚合酶链反应比较了吸烟者和非吸烟者臀部皮肤中基质金属蛋白酶1（MMP-1）的mRNA浓度。MMP-1可降解胶原蛋白，而胶原蛋白占真皮干重的至少70%。我们报告称，吸烟者皮肤中的MMP-1 mRNA明显多于非吸烟者，而金属蛋白酶组织抑制剂1（TIMP-1）或管家基因甘油醛-3-磷酸脱氢酶（GAPDH）则未观察到差异。我们认为，吸烟诱导产生的MMP-1可能在吸烟对皮肤老化的影响中起重要作用。
