猫立克次体和普氏立克次体欧洲人株及北美飞鼠株rompB基因的系统发育分析。

Phylogenetic analysis of the rompB genes of Rickettsia felis and Rickettsia prowazekii European-human and North American flying-squirrel strains.

作者信息

Moron C G, Bouyer D H, Yu X J, Foil L D, Crocquet-Valdes P, Walker D H

机构信息

Department of Pathology, University of Texas Medical Branch, Galveston 77555-0609, USA.

出版信息

Am J Trop Med Hyg. 2000 May;62(5):598-603. doi: 10.4269/ajtmh.2000.62.598.

DOI:10.4269/ajtmh.2000.62.598

PMID:11289671

Abstract

The rickettsial outer membrane protein B (rompB) gene encodes the major surface antigens of Rickettsia species. We undertook sequencing and molecular analysis of the rompB gene of Rickettsia felis and a comparison with its homologs in spotted fever group (SFG) and typhus group (TG) rickettsiae, including the complete sequences of two North American flying squirrel strains and two European human strains of Rickettsia prowazekii. We sequenced 5,226 base pairs (bp) of the R. felis rompB, encoding a protein of 1,654 amino acids. We also sequenced 5,015 bp of rompB of the flying squirrel strains, encoding a protein of 1,643 amino acids. Analysis of the R. felis rompB gene sequence showed 10-13% divergence from SFG rickettsiae and 18% divergence from the TG rickettsiae. The rompB of all sequenced strains of R. prowazekii showed an overall similarity of 99.7-99.9%.

摘要

立克次氏体外膜蛋白B（rompB）基因编码立克次氏体属的主要表面抗原。我们对立克次氏体（Rickettsia felis）的rompB基因进行了测序和分子分析，并将其与斑点热群（SFG）和斑疹伤寒群（TG）立克次氏体中的同源基因进行了比较，包括两个北美飞鼠株系和两个欧洲人源普氏立克次氏体（Rickettsia prowazekii）株系的完整序列。我们测定了猫立克次氏体rompB的5226个碱基对（bp），其编码一个含1654个氨基酸的蛋白质。我们还测定了飞鼠株系rompB的5015 bp，其编码一个含1643个氨基酸的蛋白质。猫立克次氏体rompB基因序列分析表明，与斑点热群立克次氏体有10 - 13%的差异，与斑疹伤寒群立克次氏体有18%的差异。所有测序的普氏立克次氏体株系的rompB总体相似度为99.7 - 99.9%。