Abou-Donia M B, Goldstein L B, Dechovskaia A, Bullman S, Jones K H, Herrick E A, Abdel-Rahman A A, Khan W A
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
J Toxicol Environ Health A. 2001 Apr 6;62(7):523-41. doi: 10.1080/152873901300007824.
DEET and permethrin were implicated in the development of illnesses in some veterans of the Persian Gulf War. This study was designed to investigate the effects of daily dermal application of these chemicals, alone or in combination, on the permeability of the blood-brain barrier (BBB) and blood-testes barrier (BTB) and on sensorimotor performance in male Sprague-Dawley rats. Groups of five rats were treated with a dermal daily dose of 4, 40, or 400 mg/kg DEET in ethanol or 0.013, 0.13, or 1.3 mg/kg permethrin in ethanol for 60 d. A group of 10 rats received a daily dermal dose of ethanol and served as controls. BBB permeability was assessed by injection of an iv dose of the quaternary ammonium compound [3H]hexamethonium iodide. While permethrin produced no effect on BBB permeability, DEET alone caused a decrease in BBB permeability in brainstem. A combination of DEET and permethrin significantly decreased the BBB permeability in the cortex. BTB permeability was decreased by treatment with DEET alone and in combination with permethrin. The same animals underwent a battery of functional behavior tests 30, 45, and 60 d after exposure to evaluate their sensorimotor abilities. All treatments caused a significant decline in sensorimotor performance in a dose- and time-dependent manner. These results show that daily dermal exposure to DEET, alone or in combination with permethrin, decreased BBB permeability in certain brain regions, and impaired sensorimotor performance.
在一些海湾战争退伍军人中，避蚊胺（DEET）和氯菊酯与疾病的发生有关。本研究旨在调查每日经皮单独或联合应用这些化学物质对雄性Sprague-Dawley大鼠血脑屏障（BBB）和血睾屏障（BTB）通透性以及感觉运动性能的影响。将五组大鼠每日经皮给予乙醇中4、40或400 mg/kg的避蚊胺，或乙醇中0.013、0.13或1.3 mg/kg的氯菊酯，持续60天。一组10只大鼠每日经皮给予乙醇作为对照。通过静脉注射季铵化合物[3H]碘化六甲铵来评估血脑屏障的通透性。虽然氯菊酯对血脑屏障通透性没有影响，但单独使用避蚊胺会导致脑干血脑屏障通透性降低。避蚊胺和氯菊酯联合使用会显著降低皮质的血脑屏障通透性。单独使用避蚊胺以及与氯菊酯联合使用均可降低血睾屏障通透性。在暴露后30、45和60天，对相同的动物进行一系列功能行为测试，以评估它们的感觉运动能力。所有处理均以剂量和时间依赖性方式导致感觉运动性能显著下降。这些结果表明，每日经皮暴露于避蚊胺单独或与氯菊酯联合使用，会降低某些脑区的血脑屏障通透性，并损害感觉运动性能。
