Powars D, Schroeder W A, White L
Pediatrics. 1975 May;55(5):630-5.
Accurate specific diagnosis of sickle cell disease can now be made at birth on routinely obtained cord blood samples by microcolumn chromatography. The method uses a small column of a cation ion exchange resin, CM-Sephadex, and a single developer that allows definitive rapid distinction of hemoglobin SS, AS, AC, SC, and CC, within two hours. Seventy-five samples or more per day have been analyzed by one technician in a laboratory without special precautions or equipment. In a program which has been initiated on a large, totally unpredictable obstetrical service in Los Angeles, 10,401 consecutively born infants have been studied for hemoglobin type without regard to racial origin. Three SS infants, 1 SC, 143 AS infants, and 37 AC infants as well as several with other abnormal hemoglobins have been identified without interfering with the routine operation of the delivery rooms of the obstetrical service. The diagnosis of sickle cell disease has been confirmed on subsequent examinations of the infants. The feasibility of using microcolumn chromatography as a rapid, accurate, inexpensive, and easy method for the rapid diagnosis of sickle cell disease in newborns has now been established.
现在，通过微柱色谱法对常规采集的脐带血样本进行检测，在出生时就能准确地对镰状细胞病进行特异性诊断。该方法使用一小柱阳离子交换树脂CM - 葡聚糖凝胶，以及一种单一的显影剂，能够在两小时内明确快速地区分血红蛋白SS、AS、AC、SC和CC。在一个没有特殊防护措施或设备的实验室里，一名技术人员每天能分析75个或更多的样本。在洛杉矶一项针对庞大且完全无法预测的产科服务启动的项目中，对10401名连续出生的婴儿进行了血红蛋白类型研究，而不考虑其种族出身。已识别出3名血红蛋白SS婴儿、1名血红蛋白SC婴儿、143名血红蛋白AS婴儿、37名血红蛋白AC婴儿以及几名携带其他异常血红蛋白的婴儿，且未干扰产科服务产房的常规运作。对这些婴儿随后的检查证实了镰状细胞病的诊断。现在已经证实，使用微柱色谱法作为一种快速、准确、廉价且简便的方法来快速诊断新生儿镰状细胞病是可行的。
