Microchromatography of hemoglobins. IV. An improved procedure for the detection of hemoglobins S and C at birth.

Substitution of CM-cellulose for CM-Sephadex had yielded a superior microchromatographic method for distinguishing the AS, AC, SS, SC, and CC conditions at birth. On the translucent columns of CM-Sephadex, the hemoglobin zones are somewhat diffuse. However, the compact, well-defined zones on the CM-cellulose column facilitate the interpretation of the results even though the amount of sample is only 20 per cent as great. The CM-cellulose method is as simple and rapid as the original CM-Sephadex procedure.