Arienti G, Polci A, De Cosmo A, Saccardi C, Carlini E, Palmerini C A
Istituto di Biochimica e Chimica Medica, Via del Giochetto, 06127, Perugia, Italy.
Comp Biochem Physiol B Biochem Mol Biol. 2001 Apr;128(4):661-6. doi: 10.1016/s1096-4959(00)00351-1.
The semen of several mammals contains vesicles of different composition and origin. We have recently reported on the presence of lipoprotein vesicles in stallion semen. To a certain extent, these resemble human prostasomes, but differ from them in amount and composition. These horse-semen prostasome-like vesicles may be important, not only in horse reproductive physiology, but also in view of stallion semen cryopreservation. In this paper, we have studied horse-semen prostasome-like vesicles and found that they possess less saturated fatty acid than human prostasomes. Moreover, their protein pattern (SDS-PAGE electrophoresis) shows that the 30-50-kDa fraction is less abundant in stallion vesicles. In addition, fluidity (measured as fluorescence anisotropy of diphenylhexatriene) is higher in horse prostasome-like vesicles than in human prostasomes, albeit being much lower than that of most membranes. These findings may be connected to some species-related differences in reproductive physiology: the vaginal milieu of the mare is not acidic and the deposition of semen is intrauterine in the horse but vaginal in humans.
几种哺乳动物的精液中含有成分和来源各异的小泡。我们最近报道了公马精液中存在脂蛋白小泡。在一定程度上，这些小泡类似于人类的前列腺小体，但在数量和成分上与人类前列腺小体有所不同。这些类似前列腺小体的马精液小泡可能不仅对公马生殖生理学很重要，而且鉴于公马精液的冷冻保存也很重要。在本文中，我们研究了类似前列腺小体的马精液小泡，发现它们所含的饱和脂肪酸比人类前列腺小体少。此外，它们的蛋白质图谱（SDS - PAGE电泳）显示，在公马小泡中，30 - 50 kDa部分的含量较少。另外，类似前列腺小体的马精液小泡的流动性（以二苯基己三烯的荧光各向异性来衡量）高于人类前列腺小体，尽管仍远低于大多数细胞膜的流动性。这些发现可能与生殖生理学中某些物种相关的差异有关：母马的阴道环境不是酸性的，马的精液是在子宫内沉积，而人类的精液是在阴道内沉积。
