Department of Medical Sciences, Clinical Chemistry , University Hospital , Uppsala , Sweden.
Syst Biol Reprod Med. 2013 Dec;59(6):297-303. doi: 10.3109/19396368.2013.822612. Epub 2013 Aug 2.
Release of nanometer-sized prostasomes into human and equine semen suggests essential functions in their relationships with sperm cells and the fertilization process. The two types of prostasomes displayed ultrastructural similarities, albeit the human prostasomes were somewhat larger than the stallion prostasomes. A high ratio of saturated fatty acids was characteristic for the two prostasome types. Electrophoretic separation systems revealed an equine prostasomal pattern different from that of human. The 21 distinctive low molecular weight protein spots in the 2D-gel (with no counterparts in human prostasomes) were identified via peptide mass fingerprinting, several of which may be different isoforms. Out of the three high molecular weight bands characteristic for human prostasomes (CD10, CD13, and CD26), CD10 and CD13 were retrieved in equine prostasomes. We present some new proteins of horse prostasomes not found in their human counterparts. Further studies are warranted to reveal the function of these proteins.
纳米级前列腺小体在人和马精液中的释放表明它们在与精子细胞的关系和受精过程中具有重要功能。这两种前列腺小体表现出超微结构的相似性，尽管人前列腺小体稍大于马前列腺小体。两种前列腺小体的特征是高比例的饱和脂肪酸。电泳分离系统显示马前列腺小体的模式与人类不同。通过肽质量指纹图谱鉴定了 2D 凝胶中 21 个独特的低分子量蛋白质斑点（在人类前列腺小体中没有对应物），其中一些可能是不同的同工型。在人类前列腺小体的三个高分子量带（CD10、CD13 和 CD26）中，CD10 和 CD13 在马前列腺小体中被检出。我们提出了一些在人类前列腺小体中未发现的马前列腺小体的新蛋白。进一步的研究需要揭示这些蛋白质的功能。
