Kessler-Liebscher B E, Staudenbauer W L, Hofschneider P H
Nucleic Acids Res. 1975 Jan;2(1):131-41. doi: 10.1093/nar/2.1.131.
Pulse-labeled replicative intermediates in M 13 single stranded DNA synthesis can be separated by dye-buoyant density centrifugation into two major fractions: Supercoiled molecules (RI I) containing viral strands of more than one genome length, and "relaxed" molecules (RI II) with labeled DNA chains shorter than unit length. It is postulated that RI II molecules might be formed in vivo by site-specific nicking of RF I molecules.
在M13单链DNA合成中,脉冲标记的复制中间体可通过染料浮力密度离心法分离成两个主要部分:含有多于一个基因组长度病毒链的超螺旋分子(RI I),以及标记DNA链短于单位长度的“松弛”分子(RI II)。据推测,RI II分子可能是在体内由RF I分子的位点特异性切口形成的。