Ray D S, Dueber J, Suggs S
J Virol. 1975 Aug;16(2):348-55. doi: 10.1128/JVI.16.2.348-355.1975.
Temperature-shift experiments with an Escherichia coli dnaG strain indicate a requirement for the dnaG function for M13 phage production only at an early stage of infection. Mutant cells infected at nonpermissive temperature form the parental RF (SS leads to RF) but do not replicate further. A shift to nonpermissive temperature after infection inhibits RF leads to RF replication but not RF leads to SS synthesis. The synthesis of both strands of the duplex RF was inhibited equally after a temperature shift during RF leads to RF replication. We infer that the dnaG protein is required for M13 production only during RF replication and that it is required for the synthesis of both strands of the RF.
用大肠杆菌dnaG菌株进行的温度转换实验表明，仅在感染的早期阶段，M13噬菌体的产生需要dnaG功能。在非允许温度下感染的突变细胞形成亲本复制型（单链转化为复制型），但不会进一步复制。感染后转换到非允许温度会抑制复制型到复制型的复制，但不会抑制复制型到单链的合成。在复制型到复制型的复制过程中进行温度转换后，双链复制型两条链的合成均受到同等程度的抑制。我们推断，dnaG蛋白仅在复制型复制期间是M13产生所必需的，并且它是复制型两条链的合成所必需的。
