φX174单链DNA的复制机制。IX. 对大肠杆菌dnaG蛋白的需求
Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein.
作者信息
McFadden G, Denhardt D T
出版信息
J Virol. 1974 Nov;14(5):1070-5. doi: 10.1128/JVI.14.5.1070-1075.1974.
Abstract
Escherichia coli NY73, possessing a temperature-sensitive mutation in the dnaG locus, was rendered sensitive to bacteriophage phiX174 by P1 transduction. phiX174 reproduces in this strain at 30 C but not at 40 C. All three stages of phiX174 replication, parental replicative form (RF) synthesis, RF replication, and progeny single-stranded DNA synthesis, are thermolabile in this mutant. Competition-annealing data show that both plus- and minus-strand synthesis are equally inhibited after shift up to 40 C during RF replication. We conclude that the dnaG gene product is required for the synthesis of both strands of phiX RF during RF replication and of the complementary strand and viral progeny strands during stages I and III, respectively.
摘要
大肠杆菌NY73在dnaG基因座存在温度敏感突变，通过P1转导使其对噬菌体phiX174敏感。phiX174在该菌株中于30℃时可繁殖，但在40℃时不能。phiX174复制的所有三个阶段，即亲本复制型（RF）合成、RF复制和子代单链DNA合成，在该突变体中均对温度敏感。竞争退火数据表明，在RF复制过程中温度升至40℃后，正链和负链合成均受到同等程度的抑制。我们得出结论，dnaG基因产物分别在RF复制期间phiX RF两条链的合成过程中，以及在阶段I和III期间互补链和病毒子代链的合成过程中是必需的。
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