Nielsen K, Gall D, Smith P, Kelly W, Yeo J, Kenny K, Heneghan T, McNamara S, Maher P, O'Connor J, Walsh B, Carroll J, Rojas X, Rojas F, Perez B, Wulff O, Buffoni L, Salustio E, Gregoret R, Samartino L, Dajer A, Luna-Martinez E
Canadian Food Inspection Agency, Animal Diseases Research Institute, 3851 Fallowfield Rd., Nepean, Ontario, Canada K2H 8P9.
Vet Microbiol. 2001 May 21;80(2):163-70. doi: 10.1016/s0378-1135(00)00386-2.
A fluorescence polarization assay (FPA) was used to test whole blood samples prepared by mixing blood cells from cattle without exposure to Brucella abortus (B. abortus) with sera from animals with confirmed (bacteriologically) infection. A cut-off value between negative and positive values was initially established to be 87.2mP. This value was changed to 95mP to increase assay specificity without loss of sensitivity when testing blood samples from negative animals. The FPA technology was applied to whole blood samples in the field and to stored whole blood samples using two diluent buffers. Relative sensitivity and specificity values for the FPA performed in the field, based on buffered antigen plate agglutination test and competitive enzyme immunoassay results were 95.3 and 97.3%, respectively. However, to obtain maximum sensitivity and specificity, a cut-off value of 105mP was determined for fresh whole blood samples. The relative sensitivity and specificity values of the FPA when testing stored whole blood samples were 100% each using a 95mP cut-off.The usefulness of the FPA for testing whole blood samples in the field was demonstrated.
采用荧光偏振分析（FPA）检测全血样本，这些全血样本由未接触过流产布鲁氏菌（B. abortus）的牛的血细胞与经确诊（细菌学确诊）感染动物的血清混合制备而成。最初确定阴性和阳性值之间的临界值为87.2mP。在检测阴性动物的血样时，为提高检测特异性且不损失敏感性，该值改为95mP。FPA技术应用于现场全血样本以及使用两种稀释缓冲液的储存全血样本。基于缓冲抗原平板凝集试验和竞争酶免疫分析结果，现场进行的FPA的相对敏感性和特异性值分别为95.3%和97.3%。然而，为获得最大敏感性和特异性，确定新鲜全血样本的临界值为105mP。使用95mP临界值检测储存全血样本时，FPA的相对敏感性和特异性值均为100%。证明了FPA在现场检测全血样本的实用性。
