• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
A fluorescence polarization immunoassay for the rapid detection of antibody against influenza A virus in chicken and goat sera.一种用于快速检测鸡和山羊血清中抗甲型流感病毒抗体的荧光偏振免疫分析。
J Vet Diagn Invest. 2020 Nov;32(6):887-891. doi: 10.1177/1040638720960046. Epub 2020 Oct 7.
2
Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer.基于便携式分析仪的用于检测 H5 禽流感病毒的非竞争荧光偏振免疫分析。
Anal Bioanal Chem. 2021 Jul;413(18):4619-4623. doi: 10.1007/s00216-021-03193-y. Epub 2021 Feb 5.
3
Rapid detection of anti-H5 avian influenza virus antibody by fluorescence polarization immunoassay using a portable fluorescence polarization analyzer.使用便携式荧光偏振分析仪通过荧光偏振免疫分析法快速检测抗H5禽流感病毒抗体。
Sens Actuators B Chem. 2020 Aug 1;316:128160. doi: 10.1016/j.snb.2020.128160. Epub 2020 Apr 21.
4
Simultaneous detection of avian influenza virus NP and H5 antibodies in chicken sera using a fluorescence microsphere immunoassay.使用荧光微球免疫测定法同时检测鸡血清中的禽流感病毒核蛋白（NP）和H5抗体。
Avian Dis. 2010 Mar;54(1 Suppl):668-72. doi: 10.1637/8818-040209-ResNote.1.
5
Potential Role of Nonneutralizing IgA Antibodies in Cross-Protective Immunity against Influenza A Viruses of Multiple Hemagglutinin Subtypes.非中和性 IgA 抗体在针对多种血凝素亚型流感 A 病毒的交叉保护免疫中的潜在作用。
J Virol. 2020 Jun 1;94(12). doi: 10.1128/JVI.00408-20.
6
A microsphere immunoassay for detection of antibodies to avian influenza virus.一种用于检测禽流感病毒抗体的微球免疫测定法。
J Virol Methods. 2006 Oct;137(1):88-94. doi: 10.1016/j.jviromet.2006.06.004. Epub 2006 Jul 11.
7
Novel protein microarray for the detection of avian influenza virus antibodies and simultaneous distinction of antibodies against H5 and H7 subtypes.用于检测禽流感病毒抗体并同时区分针对 H5 和 H7 亚型抗体的新型蛋白质微阵列。
Avian Pathol. 2019 Dec;48(6):528-536. doi: 10.1080/03079457.2019.1634791. Epub 2019 Jul 12.
8
Application and evaluation of RT-PCR-ELISA for the nucleoprotein and RT-PCR for detection of low-pathogenic H5 and H7 subtypes of avian influenza virus.逆转录聚合酶链反应-酶联免疫吸附测定法检测禽流感病毒核蛋白及逆转录聚合酶链反应检测低致病性H5和H7亚型禽流感病毒的应用与评价
J Vet Diagn Invest. 2004 Jan;16(1):51-6. doi: 10.1177/104063870401600109.
9
Exposure to swine H1 and H3 and avian H5 and H9 influenza a viruses among feral swine in Southern China, 2009.2009年中国南方野猪中猪H1和H3以及禽H5和H9甲型流感病毒的暴露情况
J Wildl Dis. 2013 Apr;49(2):375-80. doi: 10.7589/2012-03-079.
10
Development of a duplex Fluorescent Microsphere Immunoassay (FMIA) for the detection of antibody responses to influenza A and newcastle disease viruses.建立一种用于检测抗流感 A 病毒和新城疫病毒抗体反应的双荧光微球免疫分析（FMIA）。
J Immunol Methods. 2014 Mar;405:167-77. doi: 10.1016/j.jim.2014.02.004. Epub 2014 Feb 17.

引用本文的文献

1
Fluorescence Polarization Assay for Infection Diagnostics: A Review.荧光偏振分析在感染诊断中的应用：综述
Molecules. 2024 Oct 5;29(19):4712. doi: 10.3390/molecules29194712.

本文引用的文献

1
A brief history of bird flu.禽流感简史。
Philos Trans R Soc Lond B Biol Sci. 2019 Jun 24;374(1775):20180257. doi: 10.1098/rstb.2018.0257.
2
Geographical and Historical Patterns in the Emergences of Novel Highly Pathogenic Avian Influenza (HPAI) H5 and H7 Viruses in Poultry.家禽中新型高致病性禽流感（HPAI）H5和H7病毒出现的地理和历史模式。
Front Vet Sci. 2018 Jun 5;5:84. doi: 10.3389/fvets.2018.00084. eCollection 2018.
3
Characterization of cross-clade monoclonal antibodies against H5N1 highly pathogenic avian influenza virus and their application to the antigenic analysis of diverse H5 subtype viruses.针对H5N1高致病性禽流感病毒的跨谱系单克隆抗体的特性及其在多种H5亚型病毒抗原分析中的应用。
Arch Virol. 2017 Aug;162(8):2257-2269. doi: 10.1007/s00705-017-3350-0. Epub 2017 Apr 12.
4
Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer.利用下一代测序仪从头测序鉴定新型牛群 A 轮状病毒 G15P[14] 株引起的成年牛流行性腹泻。
Vet Microbiol. 2014 Jun 25;171(1-2):66-73. doi: 10.1016/j.vetmic.2014.03.009. Epub 2014 Mar 18.
5
A human antibody recognizing a conserved epitope of H5 hemagglutinin broadly neutralizes highly pathogenic avian influenza H5N1 viruses.一种人源抗体识别 H5 血凝素的保守表位，广泛中和高致病性禽流感 H5N1 病毒。
J Virol. 2012 Mar;86(6):2978-89. doi: 10.1128/JVI.06665-11. Epub 2012 Jan 11.
6
Fluorescence polarization immunoassay of mycotoxins: a review.真菌毒素荧光偏振免疫分析：综述。
Toxins (Basel). 2009 Dec;1(2):196-207. doi: 10.3390/toxins1020196. Epub 2009 Dec 10.
7
Fluorescence polarization assay for diagnosis of human brucellosis.用于诊断人类布鲁氏菌病的荧光偏振测定法。
J Med Microbiol. 2003 Oct;52(Pt 10):883-887. doi: 10.1099/jmm.0.05217-0.
8
Fluorescence polarization assay for the diagnosis of bovine brucellosis: adaptation to field use.用于诊断牛布鲁氏菌病的荧光偏振测定法：适应现场使用
Vet Microbiol. 2001 May 21;80(2):163-70. doi: 10.1016/s0378-1135(00)00386-2.
9
Fluorescence polarization immunoassay: detection of antibody to Brucella abortus.荧光偏振免疫测定法：流产布鲁氏菌抗体检测
Methods. 2000 Sep;22(1):71-6. doi: 10.1006/meth.2000.1038.
10
Detection and quantification of CD4(+) T cells with specificity for a new major histocompatibility complex class II-restricted influenza A virus matrix protein epitope in peripheral blood of influenza patients.检测和定量流感患者外周血中对一种新的主要组织相容性复合体II类限制性甲型流感病毒基质蛋白表位具有特异性的CD4(+) T细胞。
J Virol. 2000 Sep;74(18):8740-3. doi: 10.1128/jvi.74.18.8740-8743.2000.

一种用于快速检测鸡和山羊血清中抗甲型流感病毒抗体的荧光偏振免疫分析。

A fluorescence polarization immunoassay for the rapid detection of antibody against influenza A virus in chicken and goat sera.

机构信息

Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Japan.

Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Japan.

出版信息

J Vet Diagn Invest. 2020 Nov;32(6):887-891. doi: 10.1177/1040638720960046. Epub 2020 Oct 7.

DOI:10.1177/1040638720960046
PMID:33025860
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7649541/
Abstract

Highly pathogenic influenza A viruses (IAVs) cause substantial damage to the poultry industry. A simple and quick testing method is required for strict control of this infectious agent. The fluorescence polarization immunoassay (FPIA) is a rapid test based on antigen-antibody binding, which can detect antigen-specific antibody in the infected animal samples within a few minutes. FPIA is a one-step reaction assay that does not require a secondary antibody and complicated steps. We evaluated the usefulness of FPIA for the detection of anti-IAV antibodies, including those against internal proteins and H5 subtype HA, in sera. In the FPIA using fluorescent peptides of internal NP and M1 proteins, millipolarization units (MPUs), which increase depending on the amount of antibody, were higher in antibody-positive sera than in antibody-negative sera. Moreover, in FPIA using fluorescent recombinant H5 subtype HA proteins, anti-H5 serum gave the highest MPUs among the antisera raised in goats against individual H1-H15 subtype IAVs. Our results support the utility of FPIA for the detection of anti-IAV antibodies, especially the anti-H5 antibody.

摘要

高致病性流感病毒（IAV）对家禽业造成了巨大的破坏。需要一种简单、快速的检测方法来严格控制这种传染性病原体。荧光偏振免疫分析（FPIA）是一种基于抗原-抗体结合的快速检测方法，可在几分钟内检测感染动物样本中针对特定抗原的抗体。FPIA 是一种一步反应检测法，不需要二次抗体和复杂的步骤。我们评估了 FPIA 在检测血清中抗 IAV 抗体（包括针对内部蛋白和 H5 亚型 HA 的抗体）方面的有用性。在使用内部 NP 和 M1 蛋白的荧光肽的 FPIA 中，抗体阳性血清中的毫偏振单位（MPU）比抗体阴性血清中的更高，这取决于抗体的数量。此外，在使用荧光重组 H5 亚型 HA 蛋白的 FPIA 中，针对单个 H1-H15 亚型 IAV 产生的抗 H5 血清在产生的抗血清中给出了最高的 MPU。我们的结果支持 FPIA 在检测抗 IAV 抗体，特别是抗 H5 抗体方面的应用。