Kutchai H, Staub N C
J Gen Physiol. 1969 May;53(5):576-89. doi: 10.1085/jgp.53.5.576.
We measured the rate of oxygen transport through thin (165 micro) films of packed erythrocytes (Hb concentration = 30 g/100 ml). Under optimal conditions steady-state O(2) diffusion was nearly three times that found when the hemoglobin was prevented from acting as a carrier molecule by carbon monoxide binding or high oxygen back pressure. After each experiment we measured hemolysis and found that it averaged less than 1%. Hemolysis could not account for the facilitation, thus proving that facilitated transport of O(2) by hemoglobin can occur in red blood cells. The rate of facilitated transport was identical for Hb solutions of equal concentration to the cells. We interpret this to mean that under the conditions of our experiments the red cell membrane offers no detectable diffusion resistance to O(2) and that the mobility of Hb in intact red cells is the same as in concentrated Hb solution.
我们测量了氧气透过紧密堆积的红细胞薄膜（厚度为165微米，血红蛋白浓度 = 30克/100毫升）的传输速率。在最佳条件下，稳态氧气扩散速率几乎是通过一氧化碳结合或高氧反压阻止血红蛋白作为载体分子时所发现速率的三倍。每次实验后，我们测量了溶血情况，发现平均溶血率小于1%。溶血无法解释这种促进作用，从而证明血红蛋白在红细胞中能够促进氧气的运输。对于与细胞浓度相同的血红蛋白溶液，促进运输速率是相同的。我们据此推断，在我们的实验条件下，红细胞膜对氧气没有可检测到的扩散阻力，并且完整红细胞中血红蛋白的流动性与浓缩血红蛋白溶液中的相同。
