Lien E L, Boyle F G, Wrenn J M, Perry R W, Thompson C A, Borzelleca J F
Wyeth-Ayerst Nutritionals International, Philadelphia, PA 19101, USA.
Food Chem Toxicol. 2001 Apr;39(4):385-92. doi: 10.1016/s0278-6915(00)00142-3.
Either purified or cereal-based diets may be used for toxicity testing in rats. Purified diets have advantages in terms of flexibility of formulation to meet specific study objectives and also assurance of relatively low levels of contaminants (e.g. heavy metals and pesticides). The American Institute of Nutrition recommended that the widely used purified diet AIN-76A be replaced by two newer diets, AIN-93G (for use during rapid growth, pregnancy and lactation) and AIN-93M (maintenance diet). The present study compared AIN-76A and AIN-93G by feeding these diets for 13 weeks to male and female rats. A cereal-based diet was also included for reference purposes. The groups fed purified diets had higher serum cholesterol and triglyceride levels than the chow-fed group. An increased incidence and severity of renal tubular mineralization in the purified diet groups was not observed in this study (in contrast to other published studies where rats were fed AIN-76A). Several histopathologic observations, including eosinophilic gastritis and mucification of gastric glands of the glandular stomach, occurred at higher rates in the AIN-76A group than the other dietary treatments. Hepatocellular fatty changes occurred in the purified diet groups at a significantly higher rate than in the chow diet group. In conclusion, AIN-93G is an appropriate diet for use in rat safety evaluation studies.
纯化饲料或谷物类饲料均可用于大鼠的毒性试验。纯化饲料在配方灵活性方面具有优势，能够满足特定的研究目标，还能确保相对较低水平的污染物（如重金属和农药）。美国营养学会建议，广泛使用的纯化饲料AIN - 76A应由两种更新的饲料取代，即AIN - 93G（用于快速生长、怀孕和哺乳期）和AIN - 93M（维持饲料）。本研究通过将AIN - 76A和AIN - 93G饲料分别喂养雄性和雌性大鼠13周进行比较。还纳入了一种谷物类饲料作为参考。与喂食普通饲料的组相比，喂食纯化饲料的组血清胆固醇和甘油三酯水平更高。在本研究中未观察到纯化饲料组肾小管矿化的发生率和严重程度增加（与其他发表的喂食AIN - 76A大鼠的研究相反）。包括嗜酸性胃炎和腺胃胃腺黏液化生在内的一些组织病理学观察结果，在AIN - 76A组中的发生率高于其他饮食处理组。纯化饲料组肝细胞脂肪变性的发生率显著高于普通饲料组。总之，AIN - 93G是用于大鼠安全性评估研究的合适饲料。
