Mumcuoglu K Y, Miller J, Mumcuoglu M, Friger M, Tarshis M
Department of Parasitology, the Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
J Med Entomol. 2001 Mar;38(2):161-6. doi: 10.1603/0022-2585-38.2.161.
Green fluorescent protein-producing Escherichia coli were used to investigate the fate of bacteria in the alimentary tract of sterile grown maggots, Lucilia sericata (Meigen), using a laser scanning confocal microscope. A computer program was used to analyze the intensity of the fluorescence and to quantify the number of bacteria. The crop and the anterior midgut were the most heavily infected areas of the intestine. A significant decrease in the amount of bacteria was observed in the posterior midgut. The number of bacteria decreased even more significantly in the anterior hindgut and practically no bacteria were seen in the posterior end, near the anus. The viability of bacteria in the different gut sections was examined. It was shown that 66.7% of the crops, 52.8% of the midguts, 55.6% of the anterior hindguts, and 17.8% of posterior hindguts harbored living bacteria. In conclusion, during their passage through the digestive tract the majority of E. coli was destroyed in the midgut. Most of the remaining bacteria were killed in the hindgut, indicating that the feces were either sterile or contained only small numbers of bacteria.
利用绿色荧光蛋白产生型大肠杆菌，通过激光扫描共聚焦显微镜研究无菌饲养的丝光绿蝇（Lucilia sericata，Meigen）消化道内细菌的归宿。使用计算机程序分析荧光强度并对细菌数量进行定量。嗉囊和中肠前端是肠道中感染最严重的区域。中肠后端观察到细菌数量显著减少。前肠后端细菌数量减少更为显著，在靠近肛门的后端几乎未见细菌。检测了不同肠道段中细菌的活力。结果表明，66.7%的嗉囊、52.8%的中肠、55.6%的前肠后端和17.8%的后肠后端含有活细菌。总之，在大肠杆菌通过消化道的过程中，大部分在中肠被破坏。其余大部分细菌在后肠被杀死，这表明粪便要么无菌，要么仅含有少量细菌。
