Social Determinants of Health Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran.
Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences , Urmia, Iran.
J Med Entomol. 2022 May 11;59(3):1076-1080. doi: 10.1093/jme/tjac020.
Maggot debridement therapy (MDT) is a therapy with the medical use of sterile fly larvae of certain species, particularly those within the Calliphoridae family including green bottle fly, Lucilia sericata (Meigan, Diptera: Calliphoridae), for treating chronically infected wounds and ulcers. Lucilia sericata flies were maintained under insectary conditions, and the eggs were sterilized using three treatments: hydrogen peroxide solutions, used as a hand disinfectant (Treatment 1-T1), hydrogen peroxide, surface disinfectant (Treatment 2-T2), and SaniHigene (Treatment 3-T3) and the control (without treatment). All three treatment caused the complete sterilization of eggs, and no bacterial colonies were found on the blood agar culture. The egg hatching rate after 72 h was much higher than after 24 h. Egg mortality in hydrogen peroxide solutions, T1 and T2, was 3-4% and less than in solution T3 (13%). Owing to less mortality and more sterility of the eggs, the aforementioned solutions are suggested to be appropriate for sterility in maggot therapy.
蛆清创疗法（MDT）是一种利用某些特定物种的无菌蝇幼虫进行医学治疗的方法，特别是那些属于丽蝇科（Calliphoridae）的物种，包括绿头苍蝇、丝光绿蝇（Meigan，双翅目：丽蝇科），用于治疗慢性感染的伤口和溃疡。丝光绿蝇在昆虫饲养条件下饲养，并用三种方法对其卵进行消毒：双氧水，用作手部消毒剂（处理 1-T1）、双氧水、表面消毒剂（处理 2-T2）和 SaniHigene（处理 3-T3）以及对照（未处理）。所有三种处理方法均能完全杀灭卵，在血琼脂培养物上未发现细菌菌落。72 小时后卵的孵化率明显高于 24 小时后。在双氧水溶液 T1 和 T2 中，卵的死亡率为 3-4%，低于溶液 T3（13%）。由于死亡率较低，且卵的无菌性更高，因此建议上述溶液适用于蛆治疗中的无菌性。
