Viswanathan R, Samiyappan R
Plant Pathology Section, Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore.
Microbiol Res. 2001 Mar;155(4):309-14. doi: 10.1016/S0944-5013(01)80009-4.
Chitinase production and growth of certain fluorescent pseudomonads isolated from sugarcane rhizosphere on different subtrates were studied. When chitin was substituted for glycerol in King's B medium, 3 of the 4 strains showed enhanced bacterial multiplication. Bacterial cells grown on chitin-containing medium showed enhanced antifungal activity against Colletotrichum falcatum Went causing red rot disease in sugarcane. Chitinase production was significantly higher when chitin was amended to King's B medium. Higher chitinase production was also recorded when fluorescent pseudomonad strains were grown in the medium containing crab-shell chitin. Cell-free bacterial culture filtrate from chitin-containing medium significantly inhibited mycelial growth of the pathogen. These cell-free conditioned media contained 3 to 7 polypeptides. Western blot analysis revealed five isoforms of chitinase with molecular masses of 47, 36, 32, 20 and 18.5 kDa. A possible role of chitinases in red rot disease management is discussed.
研究了从甘蔗根际分离的某些荧光假单胞菌在不同底物上几丁质酶的产生及生长情况。当在King氏B培养基中用几丁质替代甘油时，4株菌株中的3株显示出细菌增殖增强。在含几丁质培养基上生长的细菌细胞对引起甘蔗赤腐病的镰刀炭疽菌表现出增强的抗真菌活性。当向King氏B培养基中添加几丁质时，几丁质酶的产生显著增加。当荧光假单胞菌菌株在含蟹壳几丁质的培养基中生长时，也记录到较高的几丁质酶产量。来自含几丁质培养基的无细胞细菌培养滤液显著抑制了病原体的菌丝生长。这些无细胞条件培养基含有3至7种多肽。蛋白质印迹分析显示了分子量分别为47、36、32、20和18.5 kDa的5种几丁质酶同工型。文中讨论了几丁质酶在赤腐病防治中的可能作用。
