Suganthi M, Arvinth S, Senthilkumar P
Department of Plant Physiology and Biotechnology, UPASI Tea Research Institute, Valparai, Tamilnadu 642 127 India.
Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamilnadu 600 117 India.
Physiol Mol Biol Plants. 2020 Oct;26(10):2053-2060. doi: 10.1007/s12298-020-00875-2. Epub 2020 Sep 25.
Tea ( (L.) O.Kuntze) is an industry-oriented economical crop in India. Among the sap sucking pests, tea mosquito bug () is one of the most serious pests causing heavy crop loss in tea plantation. Continuous use of chemical pesticides causes environmental pollution and health hazards besides developing pesticide residues in tea powder. The control of pests by bacterial metabolite is an alternative that may contribute to reduce or eliminate the chemical pesticide use. The use of chitinase as a biological control is an emerging field of research. In the present study, Chitinase (~ 25 kDa) was purified from C-13 strain using gel-filtration chromatography and further characterized for its optimum pH, temperature and substrate specificity. Bioefficacy of chitinase from C-13 was compared with our previously reported MP-13 chitinase against . Result concluded that, 100% and 78% mortality was observed by using MP-13 chitinase and C-13 chitinase, respectively. In future, bacterial chitinase can be utilized in eco-friendly pest management strategies.
茶树（(L.) O.Kuntze）是印度一种以产业为导向的经济作物。在吸食汁液的害虫中，茶小绿叶蝉（）是导致茶园严重减产的最主要害虫之一。持续使用化学农药除了会在茶粉中产生农药残留外，还会造成环境污染和健康危害。利用细菌代谢产物防治害虫是一种有助于减少或消除化学农药使用的替代方法。使用几丁质酶进行生物防治是一个新兴的研究领域。在本研究中，通过凝胶过滤色谱法从C-13菌株中纯化出几丁质酶（约25 kDa），并进一步对其最适pH、温度和底物特异性进行了表征。将C-13菌株的几丁质酶与我们之前报道的MP-13几丁质酶对茶小绿叶蝉的生物活性进行了比较。结果表明，使用MP-13几丁质酶和C-13几丁质酶分别观察到100%和78%的死亡率。未来，细菌几丁质酶可用于生态友好型害虫管理策略。
