Faumont Nathalie, Al Saati Talal, Brousset Pierre, Offer Claudie, Delsol Georges, Meggetto Fabienne
Unité de Physiopathologie Cellulaire et Moléculaire, UPR 2163-CNRS, CHU-Purpan, Avenue de Grande-Bretagne, 31059 Toulouse Cédex, France1.
J Gen Virol. 2001 May;82(Pt 5):1169-1174. doi: 10.1099/0022-1317-82-5-1169.
Epstein--Barr virus (EBV) is associated with Hodgkin's disease (HD). However, EBV-positive Reed--Sternberg (RS) cells and EBV-positive B lymphocytes co-exist in the same EBV-positive lymph node affected by HD. In a previous report, using total lymph node DNA, the presence of two distinct EBV strains was demonstrated, but their cellular localization (i.e. RS cells vs B lymphocytes) could not be determined. To address this question, three patients with EBV-associated HD were selected in the present study and single-cell PCR of the latent membrane protein-1 (LMP-1) gene from isolated RS cells was performed. In one case, it was clear that RS cells and B lymphocytes were infected by different EBV strains. In the two remaining cases, only one band was detected from total lymph node DNA. However, single-cell PCR showed that RS cells in each sample were infected by single EBV strains, which were different from those detected in lymphoblastoid cell lines derived from EBV-positive B lymphocytes of lymph node cell suspensions from these two patients.
爱泼斯坦-巴尔病毒（EBV）与霍奇金淋巴瘤（HD）有关。然而，EBV阳性的里德-斯腾伯格（RS）细胞和EBV阳性的B淋巴细胞共存于同一个受HD影响的EBV阳性淋巴结中。在之前的一份报告中，使用淋巴结总DNA证明了存在两种不同的EBV毒株，但无法确定它们的细胞定位（即RS细胞与B淋巴细胞）。为了解决这个问题，本研究选择了3例与EBV相关的HD患者，并对分离出的RS细胞的潜伏膜蛋白1（LMP-1）基因进行了单细胞PCR。在1例病例中，很明显RS细胞和B淋巴细胞被不同的EBV毒株感染。在其余2例病例中，从淋巴结总DNA中仅检测到一条带。然而，单细胞PCR显示每个样本中的RS细胞被单一的EBV毒株感染这些毒株与从这两名患者的淋巴结细胞悬液中EBV阳性B淋巴细胞衍生的淋巴母细胞系中检测到的毒株不同。
