Tang K, Sun X, Hu Q, Wu A, Lin C -H., Lin H -J., Twyman R M., Christou P, Feng T
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, 200433, Shanghai, People's Republic of China
Plant Sci. 2001 Apr;160(5):1035-1042. doi: 10.1016/s0168-9452(01)00351-x.
We used particle bombardment to cotransform mature seed-derived rice callus (Oryza sativa L., ssp. japonica, cv. Eyi 105) with plasmids containing the linked marker genes gusA and hpt, and the ap1 gene encoding an amphipathic protein previously shown to delay the hypersensitive response induced in non-host plants by the pathogen Pseudomonas syringae pv. syringae (Pss). Thirty-two independent lines of transgenic rice plants were regenerated, and 27 of these lines carried all three transgenes as shown by molecular analysis. A bacterial blight inoculation test was carried out on ten lines. In each case, plants carrying the ap1 gene showed enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo) race 6 at various levels. This suggests the ap1 gene could be a useful candidate for genetic engineering strategies in rice to provide bacterial blight resistance.
我们使用粒子轰击法，将含有连锁标记基因gusA和hpt以及编码一种两亲性蛋白的ap1基因的质粒，共转化到成熟种子来源的水稻愈伤组织（水稻，日本亚种，品种鄂宜105）中。此前研究表明，该两亲性蛋白可延缓丁香假单胞菌丁香致病变种（Pss）在非寄主植物中诱导的超敏反应。共再生出32个独立的转基因水稻株系，分子分析显示其中27个株系携带所有三个转基因。对10个株系进行了白叶枯病接种试验。在每种情况下，携带ap1基因的植株对水稻白叶枯病菌（Xoo）6号小种均表现出不同程度的增强抗性。这表明ap1基因可能是水稻基因工程策略中提供白叶枯病抗性的一个有用候选基因。
