Davis L, Kuttan G
Amalanagar Cancer Research Centre, Amalanagar, Thrissur, 680 553, Kerala, India.
J Ethnopharmacol. 2001 May;75(2-3):165-8. doi: 10.1016/s0378-8741(00)00404-9.
Administration of an extract of Withania somnifera was found to reduce two stage skin carcinogenesis induced by DMBA (dimethyl benzanthracene) and croton oil. Withania somnifera was administered at a concentration of (20 mg/dose/animal i.p.) consecutively on 5 days prior to DMBA administration and continued twice weekly for 10 weeks. After the 180th day of carcinogen administration, all of the animals developed papilloma in the control group whereas only six out of 12 animals developed papilloma in the treated group. A total of 11 papillomas were found in the control group while only six developed them in the Withania somnifera treated group. Enzyme analysis of skin and liver showed significant enhancement in antioxidant enzymes such as GSH, GST, Glutathione peroxides and Catalases in Withania somnifera treated group when compared with the control. The elevated level of lipid peroxide in the control group was significantly inhibited by Withania somnifera administration. These studies indicate that Withania somnifera could reduce the papilloma induced alterations to the antioxidant defense systems.
研究发现，给予印度人参提取物可减少由二甲基苯并蒽（DMBA）和巴豆油诱导的两阶段皮肤癌发生。在给予DMBA前连续5天以（20毫克/剂量/动物腹腔注射）的浓度给予印度人参，并在接下来的10周内每周两次继续给药。在给予致癌物后的第180天，对照组所有动物均出现乳头瘤，而治疗组12只动物中只有6只出现乳头瘤。对照组共发现11个乳头瘤，而印度人参治疗组只有6个出现乳头瘤。与对照组相比，印度人参治疗组皮肤和肝脏的酶分析显示抗氧化酶如谷胱甘肽（GSH）、谷胱甘肽S-转移酶（GST）、谷胱甘肽过氧化物酶和过氧化氢酶显著增强。印度人参给药显著抑制了对照组中脂质过氧化物水平的升高。这些研究表明，印度人参可以减少乳头瘤诱导的抗氧化防御系统的改变。
