刺毛黧豆提取物对锯鳞蝰蛇毒激活凝血酶原的影响。

Effects of Mucuna pruriens extract on activation of prothrombin by Echis carinatus venom.

作者信息

Guerranti R, Aguiyi J C, Errico E, Pagani R, Marinello E

机构信息

Institute of Biochemistry and Enzymology, University of Siena, Via A. Moro, 53100, Siena, Italy.

出版信息

J Ethnopharmacol. 2001 May;75(2-3):175-80. doi: 10.1016/s0378-8741(00)00401-3.

DOI:10.1016/s0378-8741(00)00401-3

PMID:11297847

Abstract

Mucuna pruriens (L.) DC has long been used as a medicinal plant by traditional healers. The validity of the claims made for this plant has also been tested scientifically. Some of its properties are probably linked to high concentrations of dopa since it is useful in the treatment of Parkinson's disease. The antisnake properties of an extract of Mucuna pruriens' seeds (MP101UJ) in vivo were recently demonstrated and one is now investigating its biochemical mechanism. Echis carinatus venom (EV) contains a mixture of proteins that affect the coagulative cascade, causing severe bleeding and haemorrhage. Here the effect of an extract of MP101UJ in prothrombin activation by EV in vitro by clotting and chromogenic assay is studied. An increase in procoagulant activity was found. This could explain the protective effect in vivo.

摘要

刺毛黧豆长期以来一直被传统治疗师用作药用植物。对这种植物所宣称的功效也已进行了科学验证。它的一些特性可能与高浓度的多巴有关，因为它对治疗帕金森病有用。最近在体内证明了刺毛黧豆种子提取物（MP101UJ）的抗蛇毒特性，目前正在研究其生化机制。锯鳞蝰蛇毒液（EV）含有影响凝血级联反应的蛋白质混合物，会导致严重出血和大出血。在此，通过凝血和显色测定法研究了MP101UJ提取物对EV在体外凝血酶原激活中的作用。发现促凝血活性增加。这可以解释其在体内的保护作用。