Ghosh S, Misro M, Das U B, Maiti R, Debnath J M, Ghosh D
Reproductive Endocrinology and Family Welfare Unit, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore -721 102, West Bengal, India.
Reprod Toxicol. 2001 Mar-Apr;15(2):221-5. doi: 10.1016/s0890-6238(01)00114-9.
Quantitative evaluation of ovarian Delta5,3beta- hydroxysteroid dehydrogenese (HSD) and 17beta - HSD activities along with radioimmunoassay of plasma levels of gonadotrophins (FSH and LH), and estradiol (E2), and quantification of different types of developing follicles and regressive follicles were noted in mature rats of the Wistar strain following treatment with cyclophosphamide at a dose of 5 mg/kg body weight/day for 28 days. A significant reduction in plasma levels of LH and E2 along with significant diminution in the activities of ovarian Delta5,3beta -HSD and 17beta- HSD were observed following cyclophosphamide treatment for 28 days without any change in the plasma level of FSH. This treatment also produced a marked degree of degeneration in different types of follicles. Coadministration of hCG at 5 IU/kg body weight/day for 28 days in the cyclophosphamide-treated group provided significant protection except with respect to plasma LH. These results suggest the possibility of an indirect action of cyclophosphamide at the level of the ovary.
对Wistar品系的成年大鼠以5mg/kg体重/天的剂量给予环磷酰胺处理28天，之后对卵巢δ5,3β-羟基类固醇脱氢酶（HSD）和17β-HSD活性进行定量评估，同时采用放射免疫分析法检测血浆促性腺激素（FSH和LH）、雌二醇（E2）水平，并对不同类型的发育卵泡和退化卵泡进行定量分析。在给予环磷酰胺处理28天后，观察到血浆LH和E2水平显著降低，同时卵巢δ5,3β-HSD和17β-HSD活性显著减弱，而血浆FSH水平无变化。该处理还导致不同类型卵泡出现明显程度的退化。在环磷酰胺处理组中，以5IU/kg体重/天的剂量同时给予hCG 28天，除血浆LH外，其他指标均得到显著保护。这些结果提示环磷酰胺可能在卵巢水平产生间接作用。
