Mannaerts B, Uilenbroek J, Schot P, De Leeuw R
Medical R&D Unit, Organon International bv, Oss, The Netherlands.
Biol Reprod. 1994 Jul;51(1):72-81. doi: 10.1095/biolreprod51.1.72.
To examine the role of FSH and LH in follicular growth and atresia, immature hypophysectomized (hypox) rats were treated twice daily for four days with a total dose either of 2.5 to 40 IU recombinant human FSH (recFSH; Org 32489) or of 8 IU recFSH supplemented with 0.2 to 5 IU hCG. RecFSH alone caused dose-dependent increases in ovarian weight and intraovarian estradiol (E2) but was unable to elevate circulating E2 levels. The number of antral follicles was also increased in a recFSH dose-dependent manner, and a gradual shift of small antral follicles to large preovulatory follicles was noted. The latter ovulated after a single bolus injection of 10 IU hCG. In comparison with follicles from hypox vehicle-treated animals, these follicles showed a diminished incidence of atresia, especially in the smallest size class of antral follicles. A total dose of > or = 10 IU recFSH increased uterine weight accompanied by endometrium proliferation. When 8 IU recFSH was supplemented with 0.2 to 5 IU hCG, ovarian weight was augmented in an hCG dose-dependent fashion, but no further increases in total number of antral follicles were noted except with the highest hCG dose given. Nevertheless, addition of relatively low doses of hCG caused considerable shifts of small follicles to large, preovulatory follicles. Furthermore, supplementation with hCG, especially low dosages of hCG (0.2 and 0.5 IU), reduced the incidence of atresia in antral follicles of all size classes. These data suggest that in the complete absence of LH activity, recFSH induces follicular growth up to the stage of mature preovulatory follicles and induces ovarian estradiol production and endometrium proliferation. The addition of small amounts of LH activity increases the percentage of healthy follicles.
为研究促卵泡激素（FSH）和促黄体生成素（LH）在卵泡生长和闭锁中的作用，对未成熟的垂体切除大鼠每日进行两次治疗，持续四天，给予2.5至40国际单位重组人FSH（recFSH；Org 32489）的总剂量，或给予8国际单位recFSH并补充0.2至5国际单位人绒毛膜促性腺激素（hCG）。单独使用recFSH会导致卵巢重量和卵巢内雌二醇（E2）呈剂量依赖性增加，但无法提高循环E2水平。窦状卵泡数量也以recFSH剂量依赖性方式增加，并且观察到小窦状卵泡逐渐向大的排卵前卵泡转变。后者在单次注射10国际单位hCG后排卵。与垂体切除后给予赋形剂处理动物的卵泡相比，这些卵泡闭锁发生率降低，尤其是在最小尺寸类别的窦状卵泡中。recFSH总剂量≥10国际单位会增加子宫重量并伴有子宫内膜增殖。当8国际单位recFSH补充0.2至5国际单位hCG时，卵巢重量以hCG剂量依赖性方式增加，但除给予最高hCG剂量外，未观察到窦状卵泡总数进一步增加。然而，添加相对低剂量的hCG会导致小卵泡大量转变为大的排卵前卵泡。此外，补充hCG，尤其是低剂量hCG（0.2和0.5国际单位），可降低所有尺寸类别的窦状卵泡闭锁发生率。这些数据表明，在完全缺乏LH活性的情况下，recFSH可诱导卵泡生长至成熟排卵前卵泡阶段，并诱导卵巢产生雌二醇和子宫内膜增殖。添加少量LH活性可增加健康卵泡的百分比。
