Shah J P, Midkiff P, Brandt P C, Sisken B F
Center for Biomedical Engineering and Department of Anatomy and Neurobiology, University of Kentucky, Lexington 40506-0070, USA.
Bioelectromagnetics. 2001 May;22(4):267-71. doi: 10.1002/bem.49.
Previous studies in our laboratory showed that neurite outgrowth in vitro and nerve regeneration in vivo were stimulated by 2 Hz, 0.3 mT (3 G) pulsed electromagnetic fields (PEMF). To learn more about the effects of PEMF on nerve cells, we exposed PC6 cells, a standard neuronal-like cell model, to the same pulsed electromagnetic fields for 2 h/day for 2 days and asked whether two different cell processes, proliferation and differentiation, were affected. The cells were also treated with a differentiating agent, nerve growth factor (NGF), to further define any interactive effects. We found that proliferation was unaffected by either PEMF or NGF alone or in combination. Differentiation, expressed as neurite outgrowth, was strongly upregulated with NGF, but this NGF response was significantly depressed in cells treated with PEMF.
我们实验室之前的研究表明，2赫兹、0.3毫特斯拉（3高斯）的脉冲电磁场（PEMF）可刺激体外神经突生长和体内神经再生。为了更深入了解PEMF对神经细胞的影响，我们将PC6细胞（一种标准的神经元样细胞模型）暴露于相同的脉冲电磁场中，每天2小时，持续2天，并研究两种不同的细胞过程——增殖和分化是否受到影响。细胞还接受了分化剂神经生长因子（NGF）处理，以进一步确定任何交互作用。我们发现，单独使用PEMF或NGF，或两者联合使用，均不影响细胞增殖。以神经突生长表示的分化在NGF作用下强烈上调，但在用PEMF处理的细胞中，这种NGF反应显著受到抑制。
