Cassán F, Bottini R, Schneider G, Piccoli P
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Universidad Nacional de Río Cuarto, Campus Universitario, 5800 Río Cuarto, Argentina.
Plant Physiol. 2001 Apr;125(4):2053-8. doi: 10.1104/pp.125.4.2053.
Azospirillum species are plant growth-promotive bacteria whose beneficial effects have been postulated to be partially due to production of phytohormones, including gibberellins (GAs). In this work, Azospirillum brasilense strain Cd and Azospirillum lipoferum strain USA 5b promoted sheath elongation growth of two single gene GA-deficient dwarf rice (Oryza sativa) mutants, dy and dx, when the inoculated seedlings were supplied with [17,17-2H2]GA20-glucosyl ester or [17,17- 2H2]GA20-glucosyl ether. Results of capillary gas chromatography-mass spectrometry analysis show that this growth was due primarily to release of the aglycone [17,17-2H2]GA20 and its subsequent 3beta-hydroxylation to [17,17-2H2]GA1 by the microorganism for the dy mutant, and by both the rice plant and microorganism for the dx mutant.
固氮螺菌属细菌是促进植物生长的细菌，其有益作用据推测部分归因于植物激素的产生，包括赤霉素（GAs）。在本研究中，当给接种的幼苗供应[17,17-2H2]GA20-葡萄糖基酯或[17,17-2H2]GA20-葡萄糖基醚时，巴西固氮螺菌Cd菌株和产脂固氮螺菌USA 5b菌株促进了两个单基因GA缺陷型矮化水稻（Oryza sativa）突变体dy和dx的叶鞘伸长生长。毛细管气相色谱-质谱分析结果表明，这种生长主要是由于糖苷配基[17,17-2H2]GA20的释放，以及微生物将其随后3β-羟基化为[17,17-2H2]GA1（对于dy突变体），以及水稻植株和微生物共同作用（对于dx突变体）。
