Chen Li, Zhang Hui, Zhao Sifeng, Xiang Benchun, Yao Zhaoqun
Key Laboratory at Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization/College of Agriculture, Shihezi University, Shihezi, 832003, China.
Institute of Agricultural Resources and Environment, Chongqing Academy of Agricultural Sciences, Chongqing, 401329, China.
Biotechnol Lett. 2021 Jun;43(6):1183-1193. doi: 10.1007/s10529-021-03114-0. Epub 2021 Mar 18.
An assay was conducted to show the comparisons the effects of nine metal ions on antagonistic metabolites (lipopeptides, siderophores and gibberellins) by Bacillus atrophaeus strain B44 using well-diffusion assays, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, chrome azurol S plus mannitol salt agar (CAS-MSA) tests, and reversed-phase high-performance liquid chromatography (RP-HPLC) analysis. This assay is also designed to demonstrate the biocontrol efficacy of B44 against cotton rhizoctoniosis using pot culture tests.
Both the lipopeptide yield and the antimicrobial activity of B44 increase with the MnSO, MgSO, CaCO, and CuSO treatments and either have no effect or decreased lipopeptide yield and antimicrobial activity with the FeSO, KHPO, KCl, KHPO and ZnSO treatments. The medium containing MgSO has no significant effect on either the lipopeptide yield or antimicrobial activity. MALDI-TOF-MS analysis shows a broad range of m/z peaks, indicating that strain B44 produces a complex mixture of iturin, surfactin, and fengycin lipopeptides. Gibberellin production by strain B44 varies greatly depending on the culture medium, and the siderophore production is not significantly affected by the culture medium. Pot tests show that lipopeptide production affects the disease control efficacy of strain B44.
The biocontrol efficacy of B. atrophaeus strain B44 is related to the lipopeptide yield. Moreover, B. atrophaeus strain B44 significantly increases the size of cotton seedlings, which is related to the GA concentration.
通过打孔扩散法、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析、铬天青S加甘露醇盐琼脂（CAS-MSA）试验以及反相高效液相色谱（RP-HPLC）分析，进行了一项试验，以比较9种金属离子对萎缩芽孢杆菌B44菌株拮抗代谢产物（脂肽、铁载体和赤霉素）的影响。该试验还旨在通过盆栽试验证明B44对棉花立枯病的生防效果。
MnSO₄、MgSO₄、CaCO₃和CuSO₄处理使B44的脂肽产量和抗菌活性均增加，而FeSO₄、KH₂PO₄、KCl、K₂HPO₄和ZnSO₄处理对脂肽产量和抗菌活性要么无影响，要么使其降低。含有MgSO₄的培养基对脂肽产量或抗菌活性均无显著影响。MALDI-TOF-MS分析显示出广泛的m/z峰，表明B44菌株产生了伊枯草菌素、表面活性素和丰原素脂肽的复杂混合物。B44菌株的赤霉素产量因培养基而异，铁载体产量不受培养基显著影响。盆栽试验表明，脂肽产量影响B44菌株的病害防治效果。
萎缩芽孢杆菌B44菌株的生防效果与脂肽产量有关。此外，萎缩芽孢杆菌B44菌株显著增加了棉花幼苗的大小，这与GA浓度有关。
