Amna Touseef, Puri Satish C, Verma V, Sharma Jai P, Khajuria Rajinder K, Musarrat Javed, Spiteller Michael, Qazi G N
Regional Research Laboratory, (CSIR), Jammu, India.
Can J Microbiol. 2006 Mar;52(3):189-96. doi: 10.1139/w05-122.
Twigs (young and old) from Nothapodytes foetida growing in the Jammu and Mahabaleshwar regions in India were used for the isolation of 52 strains of endophytic fungi and were tested for their ability to produce the anticancer alkaloid camptothecin. One of the isolates from the inner bark tissue of the N. foetida plant growing in the Jammu region of J&K state, India, was found to produce detectable quantities of camptothecin and its derivatives when grown in a semi-synthetic liquid medium. Camptothecin was identified by physicochemical analysis and further confirmed by spectroscopic studies. No camptothecin was detected in zero time cultures or in uninoculated culture broth. The maximum yield of camptothecin was 0.575 +/- 0.031 mg/100 g of dry cell mass in 96 h in shake flasks, whereas 4.96 +/- 0.73 mg/100 g of dry mass was recorded in 48 h in a bioreactor.
从印度查谟和马哈巴利普兰地区生长的印度蛇根木的嫩枝（新枝和老枝）中分离出52株内生真菌，并测试了它们产生抗癌生物碱喜树碱的能力。从印度查谟和克什米尔邦查谟地区生长的印度蛇根木植物的内皮组织中分离出的一种菌株，在半合成液体培养基中生长时，被发现能产生可检测量的喜树碱及其衍生物。通过理化分析鉴定了喜树碱，并通过光谱研究进一步证实。在零时培养物或未接种的培养液中未检测到喜树碱。在摇瓶中培养96小时，喜树碱的最大产量为0.575±0.031毫克/100克干细胞质量，而在生物反应器中培养48小时，记录到的产量为4.96±0.73毫克/100克干质量。
