Gopalakrishnan Roja, Shankar Bhavani
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
Phytomedicine. 2014 Feb 15;21(3):383-9. doi: 10.1016/j.phymed.2013.09.006. Epub 2013 Nov 16.
Camptotheca alkaloids were isolated from multiple shoot cultures of O. decumbens (0.056% dry weight) and stems of N. foetida. The cytotoxicity of the extracts and products were tested in a panel of five cell lines. Crude extract from O. decumbens (Cr-Od) and N. foetida (Cr-Nf) showed more potent cytotoxic activity as compared to the isolated camptothecin from O. decumbens (CPT-Od) and N. foetida (CPT-Nf). CPT isolated from shoot cultures contained biological activity suggesting the possibility of using this system of O. decumbens as a renewable source for the production of camptotheca alkaloids. 9-Methoxy camptothecin (9-mCPT), isolated from N. foetida, was a very effective cytotoxic agent as compared to Cr-Nf or CPT-Nf. The IC50 of 9-mCPT was 0.84, 0.32, and 0.35 μg/ml for A549, MCF7 and Jurkat cell lines and >3 μg/ml for U937. Viability assays using MTT dye were further confirmed by assessing extent of apoptosis in these cells. These findings suggest that shoot cultures of O. decumbens offer a rich alternative plant source for the anticancer compound, CPT and 9-mCPT is a more potent compound in N. foetida as compared to CPT.
喜树碱类生物碱是从平卧菊三七的多芽培养物（干重0.056%）和臭灵丹草的茎中分离得到的。提取物和产物的细胞毒性在一组五种细胞系中进行了测试。与从平卧菊三七（CPT - Od）和臭灵丹草（CPT - Nf）中分离得到的喜树碱相比，平卧菊三七（Cr - Od）和臭灵丹草（Cr - Nf）的粗提物显示出更强的细胞毒性活性。从芽培养物中分离得到的喜树碱具有生物活性，这表明有可能将平卧菊三七的这个系统用作生产喜树碱类生物碱的可再生来源。从臭灵丹草中分离得到的9 - 甲氧基喜树碱（9 - mCPT）与Cr - Nf或CPT - Nf相比，是一种非常有效的细胞毒性剂。9 - mCPT对A549、MCF7和Jurkat细胞系的IC50分别为0.84、0.32和0.35μg/ml，对U937细胞系则>3μg/ml。使用MTT染料进行的活力测定通过评估这些细胞中的凋亡程度得到了进一步证实。这些发现表明，平卧菊三七的芽培养物为抗癌化合物喜树碱提供了丰富的替代植物来源，并且与喜树碱相比，9 - mCPT在臭灵丹草中是一种更有效的化合物。
